Extraction and characterization of starch granule-associated proteins from rice that affect in vitro starch digestibility

Xiaoting Ye; Yu Zhang; Chen Qiu; Harold Corke; Zhongquan Sui

doi:10.1016/j.foodchem.2018.10.042

Extraction and characterization of starch granule-associated proteins from rice that affect in vitro starch digestibility

Xiaoting Ye, Yu Zhang, Chen Qiu, Harold Corke^*, Zhongquan Sui

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Starch granule-associated proteins (SGAPs) including granule-surface proteins and granule-channel proteins in waxy, low- and high-amylose rice starch were extracted and identified. The in vitro digestibility of starch was investigated before and after the extraction of granule-channel proteins or total SGAPs. The results showed that 10 types of major differentially expressed proteins (DEPs) including 14-3-3-like protein and ribosomal protein were found among starches. In addition, the lack of only granule-channel proteins or total SGAPs led to significant and different changes in the levels of rapidly digestible starch, slowly digestible starch and resistant starch. Possible mechanisms are related to the accessibility of amylase into starch granules and structural properties of SGAPs. This study provides more information about DEPs in rice starch with different amylose content and supports further study on the relationship between SGAPs and in vitro starch digestibility.

Original language	English
Pages (from-to)	754-760
Number of pages	7
Journal	Food Chemistry
Volume	276
DOIs	https://doi.org/10.1016/j.foodchem.2018.10.042
State	Published - 15 Mar 2019
Externally published	Yes

Keywords

Amylose content
In vitro starch digestibility
Rice starch
Starch granule-associated proteins (SGAPs)

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10.1016/j.foodchem.2018.10.042

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title = "Extraction and characterization of starch granule-associated proteins from rice that affect in vitro starch digestibility",

abstract = "Starch granule-associated proteins (SGAPs) including granule-surface proteins and granule-channel proteins in waxy, low- and high-amylose rice starch were extracted and identified. The in vitro digestibility of starch was investigated before and after the extraction of granule-channel proteins or total SGAPs. The results showed that 10 types of major differentially expressed proteins (DEPs) including 14-3-3-like protein and ribosomal protein were found among starches. In addition, the lack of only granule-channel proteins or total SGAPs led to significant and different changes in the levels of rapidly digestible starch, slowly digestible starch and resistant starch. Possible mechanisms are related to the accessibility of amylase into starch granules and structural properties of SGAPs. This study provides more information about DEPs in rice starch with different amylose content and supports further study on the relationship between SGAPs and in vitro starch digestibility.",

keywords = "Amylose content, In vitro starch digestibility, Rice starch, Starch granule-associated proteins (SGAPs)",

author = "Xiaoting Ye and Yu Zhang and Chen Qiu and Harold Corke and Zhongquan Sui",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2019",

month = mar,

day = "15",

doi = "10.1016/j.foodchem.2018.10.042",

language = "英语",

volume = "276",

pages = "754--760",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "Elsevier Ltd.",

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TY - JOUR

T1 - Extraction and characterization of starch granule-associated proteins from rice that affect in vitro starch digestibility

AU - Ye, Xiaoting

AU - Zhang, Yu

AU - Qiu, Chen

AU - Corke, Harold

AU - Sui, Zhongquan

PY - 2019/3/15

Y1 - 2019/3/15

N2 - Starch granule-associated proteins (SGAPs) including granule-surface proteins and granule-channel proteins in waxy, low- and high-amylose rice starch were extracted and identified. The in vitro digestibility of starch was investigated before and after the extraction of granule-channel proteins or total SGAPs. The results showed that 10 types of major differentially expressed proteins (DEPs) including 14-3-3-like protein and ribosomal protein were found among starches. In addition, the lack of only granule-channel proteins or total SGAPs led to significant and different changes in the levels of rapidly digestible starch, slowly digestible starch and resistant starch. Possible mechanisms are related to the accessibility of amylase into starch granules and structural properties of SGAPs. This study provides more information about DEPs in rice starch with different amylose content and supports further study on the relationship between SGAPs and in vitro starch digestibility.

AB - Starch granule-associated proteins (SGAPs) including granule-surface proteins and granule-channel proteins in waxy, low- and high-amylose rice starch were extracted and identified. The in vitro digestibility of starch was investigated before and after the extraction of granule-channel proteins or total SGAPs. The results showed that 10 types of major differentially expressed proteins (DEPs) including 14-3-3-like protein and ribosomal protein were found among starches. In addition, the lack of only granule-channel proteins or total SGAPs led to significant and different changes in the levels of rapidly digestible starch, slowly digestible starch and resistant starch. Possible mechanisms are related to the accessibility of amylase into starch granules and structural properties of SGAPs. This study provides more information about DEPs in rice starch with different amylose content and supports further study on the relationship between SGAPs and in vitro starch digestibility.

KW - Amylose content

KW - In vitro starch digestibility

KW - Rice starch

KW - Starch granule-associated proteins (SGAPs)

UR - http://www.scopus.com/inward/record.url?scp=85055151367&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2018.10.042

DO - 10.1016/j.foodchem.2018.10.042

M3 - 文章

C2 - 30409658

AN - SCOPUS:85055151367

SN - 0308-8146

VL - 276

SP - 754

EP - 760

JO - Food Chemistry

JF - Food Chemistry

ER -

Extraction and characterization of starch granule-associated proteins from rice that affect in vitro starch digestibility

Abstract

Keywords

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