Examination of oral tissues by Fourier transform spectral imaging fluorescence

Michal Fisher*, Liviu Feller, Israel Schechter

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Fourier transform imaging spectroscopy was combined with fluorescence microscopy and a cooled CCD detector for examination of human oral tissues. Oral tissue fragments, obtained from patients, were irradiated at 365 nm by a mercury lamp through the microscope objectives. Microscope images were transferred to an imaging Fourier transform spectrometer and to a CCD camera for simultaneous recording of the fluorescence spectra at each image pixel. Detailed information was observed at a microscopic resolution. Oral tissue fragments were also treated with aluminum phthalocyanine tetrasulfonatre (AlPcS4) prior to irradiation and imaging. Since the latter is preferentially retained in proliferating vascular tissue such as oral tumors, its effect upon the fluorescence imaging is of practical importance. AlPcS4 is highly soluble in biological solutions and has a strong absorbance at our excitation wavelength and a strong emission peak at λ = 680 nm; therefore, it was found suitable for detection of malignant tumors by this method. It was found that the proposed spectral imaging method, when combined with fluorescence labeling, allows for direct, in vivo, medical examination of oral tissues with detailed spatial resolution.

Original languageEnglish
Pages (from-to)11-16
Number of pages6
JournalInstrumentation Science and Technology
Volume29
Issue number1
DOIs
StatePublished - Jan 2001
Externally publishedYes

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