TY - JOUR
T1 - Evaluation of microbial community composition in thermophilic methane-producing incubation of production water from a high-temperature oil reservoir
AU - Zhou, Fang
AU - Mbadinga, Serge Maurice
AU - Liu, Jin Feng
AU - Gu, Ji Dong
AU - Mu, Bo Zhong
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant No. 41073055) and the NSFC/RGC Joint Research Fund (No. 41161160560).
PY - 2013/9/1
Y1 - 2013/9/1
N2 - Investigation of petroleum microbes is fundamental for the development and utilization of oil reservoirs microbial resources, and also provides great opportunities for research and development of bio-energy. Production water from a high-temperature oil reservoir was incubated anaerobically at 55°C for more than 400 days without amendment of any nutrients. Over the time of incubation, about 1.6 mmol of methane and up to 107 mol of hydrogen (H 2) were detected in the headspace. Methane formation indicated that methanogenesis was likely the predominant process in spite of the presence of 23.4 mM SO2-4 in the production water. Microbial community composition of the incubation was characterized by means of 16S rRNA gene clone libraries construction. Bacterial composition changed from Pseudomonales as the dominant population initially to Hydrogenophilales-related microorganisms affiliated to Petrobacter spp. closely. After 400 days of incubation, other bacterial members detected were related to Anareolineales, β-, γ-, and δ-Proteobacteria. The archaeal composition of the original production water was essentially composed of obligate acetoclastic methanogens of the genus Methanosaeta, but the incubation was predominantly composed of CO 2-reducing methanogens of the genus Methanothermobacter and Crenarchaeotes-related microorganisms. Our results suggest that methanogenesis could be more active than expected in oil reservoir environments and methane formation from CO2-reduction played a significant role in the methanogenic community. This conclusion is consistent with the predominant role played by H2-oxidizing methanogens in the methanogenic conversion of organic matter in high-temperature petroleum reservoirs.
AB - Investigation of petroleum microbes is fundamental for the development and utilization of oil reservoirs microbial resources, and also provides great opportunities for research and development of bio-energy. Production water from a high-temperature oil reservoir was incubated anaerobically at 55°C for more than 400 days without amendment of any nutrients. Over the time of incubation, about 1.6 mmol of methane and up to 107 mol of hydrogen (H 2) were detected in the headspace. Methane formation indicated that methanogenesis was likely the predominant process in spite of the presence of 23.4 mM SO2-4 in the production water. Microbial community composition of the incubation was characterized by means of 16S rRNA gene clone libraries construction. Bacterial composition changed from Pseudomonales as the dominant population initially to Hydrogenophilales-related microorganisms affiliated to Petrobacter spp. closely. After 400 days of incubation, other bacterial members detected were related to Anareolineales, β-, γ-, and δ-Proteobacteria. The archaeal composition of the original production water was essentially composed of obligate acetoclastic methanogens of the genus Methanosaeta, but the incubation was predominantly composed of CO 2-reducing methanogens of the genus Methanothermobacter and Crenarchaeotes-related microorganisms. Our results suggest that methanogenesis could be more active than expected in oil reservoir environments and methane formation from CO2-reduction played a significant role in the methanogenic community. This conclusion is consistent with the predominant role played by H2-oxidizing methanogens in the methanogenic conversion of organic matter in high-temperature petroleum reservoirs.
KW - High-temperature oil reservoir
KW - Hydrogenotrophic methanogenesis
KW - Microbial community
KW - Production water
KW - Thermophilic incubation
UR - http://www.scopus.com/inward/record.url?scp=84888332417&partnerID=8YFLogxK
U2 - 10.1080/09593330.2013.786135
DO - 10.1080/09593330.2013.786135
M3 - 文章
C2 - 24527630
AN - SCOPUS:84888332417
SN - 0959-3330
VL - 34
SP - 2681
EP - 2689
JO - Environmental Technology (United Kingdom)
JF - Environmental Technology (United Kingdom)
IS - 18
ER -