Enhancing butanol tolerance and preventing degeneration in Clostridium acetobutylicum by 1-butanol-glycerol storage during long-term preservation

Xiaobo Liu, Qiuya Gu, Chunli Liao, Xiaobin Yu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

To enhance butanol tolerance and prevent productive degeneration in Clostridium acetobutylicum during long-term preservation, a novel approach called 1-butanol-glycerol storage was developed in this study. The viability, butanol tolerance and productivity of cells were investigated under each condition of storage within 12 months, respectively. The results suggested that the optimal condition of storage was in the solution containing 16 g L-1 butanol coupled with 200 g L-1 glycerol at 37 °C not -20 °C or 4 °C. Under this condition, C. acetobutylicum D64 primarily obtained the cell survival rate of 80% after 12 months. Unexpectedly, these cells still got an enhanced butanol tolerance of 32 g L-1 which was ~1 fold higher than the wild-type with butanol tolerance of 16 g L-1. Moreover, the butanol production has also got a little of increase but not dropped compared to the control. These results indicated this culture preservation method be available for enhancing butanol tolerance and preventing productive degeneration of C. acetobutylicum.

Original languageEnglish
Pages (from-to)192-197
Number of pages6
JournalBiomass and Bioenergy
Volume69
DOIs
StatePublished - 1 Oct 2014
Externally publishedYes

Keywords

  • 1-Butanol-glycerol solution (BGS)
  • Biofuel
  • Butanol tolerance
  • Clostridium acetobutylicum
  • Long-term preservation

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