Enhancement of butanol tolerance and butanol yield in Clostridium acetobutylicum mutant NT642 obtained by nitrogen ion beam implantation

TY - JOUR

T1 - Enhancement of butanol tolerance and butanol yield in Clostridium acetobutylicum mutant NT642 obtained by nitrogen ion beam implantation

AU - Liu, Xiao Bo

AU - Gu, Qiu Ya

AU - Yu, Xiao Bin

AU - Luo, Wei

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (Grant No. 21176105), Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (Grant No. KLIB-KF201105), the Fundamental Research Funds for the Central Universities (Grant No. JUSRP111A24), the 111 Project (No. 111-2-06) and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

PY - 2012/12

Y1 - 2012/12

N2 - As a promising alternative biofuel, biobutanol can be produced through acetone/butanol/ethanol (ABE) fermentation. Currently, ABE fermentation is still a small-scale industry due to its low production and high input cost. Moreover, butanol toxicity to the Clostridium fermentation host limits the accumulation of butanol in the fermentation broth. The wild-type Clostridium acetobutylicum D64 can only produce about 13 g butanol/L and tolerates less than 2% (v/v) butanol. To improve the tolerance of C. acetobutylicum D64 for enhancing the production of butanol, nitrogen ion beam implantation was employed and finally five mutants with enhanced butanol tolerance were obtained. Among these, the most butanol tolerant mutant C. acetobutylicum NT642 can tolerate above 3% (v/v) butanol while the wide-type strain can only withstand 2% (v/v). In batch fermentation, the production of butanol and ABE yield of C. acetobutylicum NT642 was 15. 4 g/L and 22. 3 g/L, respectively, which were both higher than those of its parental strain and the other mutants using corn or cassava as substrate. Enhancing butanol tolerance is a great precondition for obtaining a hyper-yield producer. Nitrogen ion beam implantation could be a promising biotechnology to improve butanol tolerance and production of the host strain C. acetobutylicum.

AB - As a promising alternative biofuel, biobutanol can be produced through acetone/butanol/ethanol (ABE) fermentation. Currently, ABE fermentation is still a small-scale industry due to its low production and high input cost. Moreover, butanol toxicity to the Clostridium fermentation host limits the accumulation of butanol in the fermentation broth. The wild-type Clostridium acetobutylicum D64 can only produce about 13 g butanol/L and tolerates less than 2% (v/v) butanol. To improve the tolerance of C. acetobutylicum D64 for enhancing the production of butanol, nitrogen ion beam implantation was employed and finally five mutants with enhanced butanol tolerance were obtained. Among these, the most butanol tolerant mutant C. acetobutylicum NT642 can tolerate above 3% (v/v) butanol while the wide-type strain can only withstand 2% (v/v). In batch fermentation, the production of butanol and ABE yield of C. acetobutylicum NT642 was 15. 4 g/L and 22. 3 g/L, respectively, which were both higher than those of its parental strain and the other mutants using corn or cassava as substrate. Enhancing butanol tolerance is a great precondition for obtaining a hyper-yield producer. Nitrogen ion beam implantation could be a promising biotechnology to improve butanol tolerance and production of the host strain C. acetobutylicum.

KW - Clostridium acetobutylicum

KW - butanol tolerance

KW - butanol yield

KW - fermentation

KW - nitrogen ion beam implantation

UR - http://www.scopus.com/inward/record.url?scp=84871762168&partnerID=8YFLogxK

U2 - 10.1007/s12275-012-2289-9

DO - 10.1007/s12275-012-2289-9

M3 - 文章

C2 - 23274990

AN - SCOPUS:84871762168

SN - 1225-8873

VL - 50

SP - 1024

EP - 1028

JO - Journal of Microbiology

JF - Journal of Microbiology

IS - 6

ER -