Engineering synergetic CO2-fixing pathways for malate production

Guipeng Hu, Jie Zhou, Xiulai Chen, Yuanyuan Qian, Cong Gao, Liang Guo, Peng Xu, Wei Chen, Jian Chen, Yin Li, Liming Liu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


Increasing the microbial CO2-fixing efficiency often requires supplying sufficient ATP and redirecting carbon flux for the production of metabolites. However, addressing these two issues concurrently remains a challenge. Here, we present a combinational strategy based on a synergetic CO2-fixing pathway that combines an ATP-generating carboxylation reaction in the central metabolic pathway with the ATP-consuming RuBisCO shunt in the carbon fixation pathway. This strategy provides enough ATP to improve the efficiency of CO2 fixation and simultaneously rewires the CO2-fixing pathway to the central metabolic pathway for the biosynthesis of chemicals. We demonstrate the application of this strategy by increasing the CO2-fixing rate and malate production in the autotroph Synechococcus elongatus by 110% and to 260 μM respectively, as well as increasing these two factors in the heterotrophic CO2-fixing Escherichia coli by 870% and to 387 mM respectively.

Original languageEnglish
Pages (from-to)496-504
Number of pages9
JournalMetabolic Engineering
StatePublished - May 2018
Externally publishedYes


  • ATP balance
  • CO fixation
  • Malate production
  • Pathway engineering


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