Engineering a Bi-conical microchip as vascular stenosis model

TY - JOUR

T1 - Engineering a Bi-conical microchip as vascular stenosis model

AU - Li, Yan

AU - Wang, Jianchun

AU - Wan, Wei

AU - Chen, Chengmin

AU - Wang, Xueying

AU - Zhao, Pei

AU - Hou, Yanjin

AU - Tian, Hanmei

AU - Wang, Jianmei

AU - Nandakumar, Krishnaswamy

AU - Wang, Liqiu

N1 - Publisher Copyright: © 2019 by the authors.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Vascular stenosis is always associated with hemodynamic changes, especially shear stress alterations. Herein, bi-conical shaped microvessels were developed through flexibly and precisely controlled templated methods for hydrogel blood-vessel-like microchip. The blood-vessel-like microvessels demonstrated tunable dimensions, perfusable ability, and good cytocompatibility. The microchips showed blood-vessel-like lumens through fine embeddedness of human umbilical vein endothelial cells (HUVECs) on the interior surface of hydrogel microchannels, which closely reproduced the morphology and functions of human blood vessels. In the gradual narrowing region of bi-conical shape, fluid flow generated wall shear stress, which caused cell morphology variations. Wall shear rates at the gradual narrowing region were simulated by FLUENT software. The results showed that our microchannels qualified for performance as a vascular stenosis-like model in evaluating blood hydrodynamics. In general, our blood-vessel-on-a-chip could offer potential applications in the prevention, diagnosis, and therapy of arterial thrombosis.

AB - Vascular stenosis is always associated with hemodynamic changes, especially shear stress alterations. Herein, bi-conical shaped microvessels were developed through flexibly and precisely controlled templated methods for hydrogel blood-vessel-like microchip. The blood-vessel-like microvessels demonstrated tunable dimensions, perfusable ability, and good cytocompatibility. The microchips showed blood-vessel-like lumens through fine embeddedness of human umbilical vein endothelial cells (HUVECs) on the interior surface of hydrogel microchannels, which closely reproduced the morphology and functions of human blood vessels. In the gradual narrowing region of bi-conical shape, fluid flow generated wall shear stress, which caused cell morphology variations. Wall shear rates at the gradual narrowing region were simulated by FLUENT software. The results showed that our microchannels qualified for performance as a vascular stenosis-like model in evaluating blood hydrodynamics. In general, our blood-vessel-on-a-chip could offer potential applications in the prevention, diagnosis, and therapy of arterial thrombosis.

KW - Bi-conical

KW - Blood-vessel-like

KW - Microchip

KW - Vascular stenosis

KW - Wall shear rate

UR - http://www.scopus.com/inward/record.url?scp=85075563140&partnerID=8YFLogxK

U2 - 10.3390/mi10110790

DO - 10.3390/mi10110790

M3 - 文章

AN - SCOPUS:85075563140

SN - 2072-666X

VL - 10

JO - Micromachines

JF - Micromachines

IS - 11

M1 - 790

ER -