Elucidation of n-butyl benzyl phthalate biodegradation using high-performance liquid chromatography and gas chromatography-mass spectrometry

Xiang Rong Xu*, Hua Bin Li, Ji Dong Gu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

n-Butyl benzyl phthalate (BBP) is an endocrinedisrupting chemical. A bacterium species capable of using BBP as the sole source of carbon and energy was isolated from mangrove sediment. Effects of BBP concentration, pH, temperature, and salinity on BBP biodegradation were studied. The optimum pH, temperature, and salinity for the BBP biodegradation were 7.0, 37°C, and 15 g L-1, respectively. BBP was completely degraded within 6 days under optimum conditions, and the biodegradation of BBP could be fitted to a first-order kinetic model. The major metabolites of BBP biodegradation were identified as mono-butyl phthalate, mono-benzyl phthalate, phthalic acid, and benzoic acid by using high-performance liquid chromatography and gas chromatography-mass spectrometry. A preliminary metabolic pathway was proposed for the biodegradation of BBP.

Original languageEnglish
Pages (from-to)370-375
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume386
Issue number2
DOIs
StatePublished - Oct 2006
Externally publishedYes

Keywords

  • Biodegradation
  • Gas chromatography-mass spectrometry
  • High-performance liquid chromatography
  • N-Butyl benzyl phthalate

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