Degradation of dimethyl isophthalate by Viarovorax paradoxus strain T4 isolated from deep-ocean sediment of the South China Sea

Y. P. Wang, J. D. Gu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Viarovorax paradoxus T4 strain was isolated from deep-ocean sediment and demonstrated to be able to degrade dimethyl isophthalate (DMI). When DMI was utilized as the sole source of carbon and energy, it was transformed by hydrolysis initially, forming monomethyl isophthalate (MMI) and isophthalate acid (IA) as degradation intermediates. DMI and MMI were completely transformed to MMI and IA in about 100 h, respectively. Degradation of IA was completed in about 55 h. Analysis of total organic carbon in the culture medium confirmed that more than 80% of the substrate carbon was mineralized. Bacterial esterase induced by a range of substrates could be assessed using p-nitrophenyl acetate as the common substrate using crude enzyme preparation. The decreasing trend of Km values derived from the Michaelis-Menten equation was dimethyl phthalate (DMP) > monomethyl phthalate (MMP) > dimethyl terephthalate (DMT) > Liver esterase > DMI > MMI > monomethyl terephthalate (MMT), indicating that higher Km values were obtained by di-esters than mono-ester and the esters induced by terephthalate esters showed the highest activity. This investigation suggests that biochemical pathways for phthalate esters share many common characteristics and the esterases induced by different substrates are highly specific.

Original languageEnglish
Pages (from-to)236-247
Number of pages12
JournalHuman and Ecological Risk Assessment (HERA)
Volume12
Issue number2
DOIs
StatePublished - Apr 2006
Externally publishedYes

Keywords

  • Biochemical pathway
  • Dimethyl isophthalate
  • Esterase
  • Isophthalate
  • Monoisophthalate
  • Plasticiser

Fingerprint Dive into the research topics of 'Degradation of dimethyl isophthalate by Viarovorax paradoxus strain T4 isolated from deep-ocean sediment of the South China Sea'. Together they form a unique fingerprint.

Cite this