Conserved eukaryotic fusogens can fuse viral envelopes to cells

Ori Avinoam; Karen Fridman; Clari Valansi; Inbal Abutbul; Tzviya Zeev-Ben-Mordehai; Ulrike E. Maurer; Amir Sapir; Dganit Danino; Kay Grünewald; Judith M. White; Benjamin Podbilewicz

doi:10.1126/science.1202333

Conserved eukaryotic fusogens can fuse viral envelopes to cells

Ori Avinoam, Karen Fridman, Clari Valansi, Inbal Abutbul, Tzviya Zeev-Ben-Mordehai, Ulrike E. Maurer, Amir Sapir, Dganit Danino, Kay Grünewald, Judith M. White, Benjamin Podbilewicz^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Caenorhabditis elegans proteins AFF-1 and EFF-1 [C. elegans fusion family (CeFF) proteins] are essential for developmental cell-to-cell fusion and can merge insect cells. To study the structure and function of AFF-1, we constructed vesicular stomatitis virus (VSV) displaying AFF-1 on the viral envelope, substituting the native fusogen VSV glycoprotein. Electron microscopy and tomography revealed that AFF-1 formed distinct supercomplexes resembling pentameric and hexameric "flowers" on pseudoviruses. Viruses carrying AFF-1 infected mammalian cells only when CeFFs were on the target cell surface. Furthermore, we identified fusion family (FF) proteins within and beyond nematodes, and divergent members from the human parasitic nematode Trichinella spiralis and the chordate Branchiostoma floridae could also fuse mammalian cells. Thus, FF proteins are part of an ancient family of cellular fusogens that can promote fusion when expressed on a viral particle.

Original language	English
Pages (from-to)	589-592
Number of pages	4
Journal	Science
Volume	332
Issue number	6029
DOIs	https://doi.org/10.1126/science.1202333
State	Published - 29 Apr 2011
Externally published	Yes

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title = "Conserved eukaryotic fusogens can fuse viral envelopes to cells",

abstract = "Caenorhabditis elegans proteins AFF-1 and EFF-1 [C. elegans fusion family (CeFF) proteins] are essential for developmental cell-to-cell fusion and can merge insect cells. To study the structure and function of AFF-1, we constructed vesicular stomatitis virus (VSV) displaying AFF-1 on the viral envelope, substituting the native fusogen VSV glycoprotein. Electron microscopy and tomography revealed that AFF-1 formed distinct supercomplexes resembling pentameric and hexameric {"}flowers{"} on pseudoviruses. Viruses carrying AFF-1 infected mammalian cells only when CeFFs were on the target cell surface. Furthermore, we identified fusion family (FF) proteins within and beyond nematodes, and divergent members from the human parasitic nematode Trichinella spiralis and the chordate Branchiostoma floridae could also fuse mammalian cells. Thus, FF proteins are part of an ancient family of cellular fusogens that can promote fusion when expressed on a viral particle.",

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doi = "10.1126/science.1202333",

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AU - Avinoam, Ori

AU - Fridman, Karen

AU - Valansi, Clari

AU - Abutbul, Inbal

AU - Zeev-Ben-Mordehai, Tzviya

AU - Maurer, Ulrike E.

AU - Sapir, Amir

AU - Danino, Dganit

AU - Grünewald, Kay

AU - White, Judith M.

AU - Podbilewicz, Benjamin

PY - 2011/4/29

Y1 - 2011/4/29

N2 - Caenorhabditis elegans proteins AFF-1 and EFF-1 [C. elegans fusion family (CeFF) proteins] are essential for developmental cell-to-cell fusion and can merge insect cells. To study the structure and function of AFF-1, we constructed vesicular stomatitis virus (VSV) displaying AFF-1 on the viral envelope, substituting the native fusogen VSV glycoprotein. Electron microscopy and tomography revealed that AFF-1 formed distinct supercomplexes resembling pentameric and hexameric "flowers" on pseudoviruses. Viruses carrying AFF-1 infected mammalian cells only when CeFFs were on the target cell surface. Furthermore, we identified fusion family (FF) proteins within and beyond nematodes, and divergent members from the human parasitic nematode Trichinella spiralis and the chordate Branchiostoma floridae could also fuse mammalian cells. Thus, FF proteins are part of an ancient family of cellular fusogens that can promote fusion when expressed on a viral particle.

AB - Caenorhabditis elegans proteins AFF-1 and EFF-1 [C. elegans fusion family (CeFF) proteins] are essential for developmental cell-to-cell fusion and can merge insect cells. To study the structure and function of AFF-1, we constructed vesicular stomatitis virus (VSV) displaying AFF-1 on the viral envelope, substituting the native fusogen VSV glycoprotein. Electron microscopy and tomography revealed that AFF-1 formed distinct supercomplexes resembling pentameric and hexameric "flowers" on pseudoviruses. Viruses carrying AFF-1 infected mammalian cells only when CeFFs were on the target cell surface. Furthermore, we identified fusion family (FF) proteins within and beyond nematodes, and divergent members from the human parasitic nematode Trichinella spiralis and the chordate Branchiostoma floridae could also fuse mammalian cells. Thus, FF proteins are part of an ancient family of cellular fusogens that can promote fusion when expressed on a viral particle.

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SN - 0036-8075

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SP - 589

EP - 592

JO - Science

JF - Science

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ER -

Conserved eukaryotic fusogens can fuse viral envelopes to cells

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