TY - JOUR
T1 - Community shift of ammonia-oxidizing bacteria along an anthropogenic pollution gradient from the Pearl River Delta to the South China Sea
AU - Cao, Huiluo
AU - Hong, Yiguo
AU - Li, Meng
AU - Gu, Ji Dong
N1 - Funding Information:
Acknowledgments The research was supported by a Ph.D. studentship (H-LC) from The University of Hong Kong and in part by the grants from Agriculture, Fisheries and Conservation Department of the Hong Kong Government (J-DG). Ms. Jessie Lai was thanked for the kindly laboratory assistance during the research conducted at The University of Hong Kong.
PY - 2012/4
Y1 - 2012/4
N2 - The phylogenetic diversity and abundance of ammonia-oxidizing beta-proteobacteria (beta-AOB) was analyzed along an anthropogenic pollution gradient from the coastal Pearl River Delta to the South China Sea using the ammonia monooxygenase subunit A (amoA) gene. Along the gradient from coastal to the open ocean, the phylogenetic diversity of the dominant genus changed from Nitrosomonas to Nitrosospira, indicating the niche specificity by these two genera as both salinity and anthropogenic influence were major factors involved. The diversity of bacterial amoA gene was also variable along the gradient, with the highest in the deep-sea sediments, followed by the marshes sediments and the lowest in the coastal areas. Within the Nitrosomonas-related clade, four distinct lineages were identified including a putative new one (A5-16) from the different sites over the large geographical area. In the Nitrosospira-related clade, the habitat-specific lineages to the deep-sea and coastal sediments were identified. This study also provides strong support that Nitrosomonas genus, especially Nitrosomonas oligotropha lineage (6a) could be a potential bio-indicator species for pollution or freshwater/wastewater input into coastal environments. A suite of statistical analyses used showed that water depth and temperature were major factors shaping the community structure of beta-AOB in this study area.
AB - The phylogenetic diversity and abundance of ammonia-oxidizing beta-proteobacteria (beta-AOB) was analyzed along an anthropogenic pollution gradient from the coastal Pearl River Delta to the South China Sea using the ammonia monooxygenase subunit A (amoA) gene. Along the gradient from coastal to the open ocean, the phylogenetic diversity of the dominant genus changed from Nitrosomonas to Nitrosospira, indicating the niche specificity by these two genera as both salinity and anthropogenic influence were major factors involved. The diversity of bacterial amoA gene was also variable along the gradient, with the highest in the deep-sea sediments, followed by the marshes sediments and the lowest in the coastal areas. Within the Nitrosomonas-related clade, four distinct lineages were identified including a putative new one (A5-16) from the different sites over the large geographical area. In the Nitrosospira-related clade, the habitat-specific lineages to the deep-sea and coastal sediments were identified. This study also provides strong support that Nitrosomonas genus, especially Nitrosomonas oligotropha lineage (6a) could be a potential bio-indicator species for pollution or freshwater/wastewater input into coastal environments. A suite of statistical analyses used showed that water depth and temperature were major factors shaping the community structure of beta-AOB in this study area.
KW - Ammonia monooxygenase α-subunit (amoA) gene
KW - Ammonia-oxidizing bacteria (AOB)
KW - Anthropogenic pollution gradient
KW - South China Sea
UR - http://www.scopus.com/inward/record.url?scp=84858438521&partnerID=8YFLogxK
U2 - 10.1007/s00253-011-3636-1
DO - 10.1007/s00253-011-3636-1
M3 - 文章
C2 - 22005744
AN - SCOPUS:84858438521
SN - 0175-7598
VL - 94
SP - 247
EP - 259
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 1
ER -