Co-occurrence of nitrite-dependent anaerobic ammonium and methane oxidation processes in subtropical acidic forest soils

TY - JOUR

T1 - Co-occurrence of nitrite-dependent anaerobic ammonium and methane oxidation processes in subtropical acidic forest soils

AU - Meng, Han

AU - Wang, Yong Feng

AU - Chan, Ho Wang

AU - Wu, Ruo Nan

AU - Gu, Ji Dong

N1 - Publisher Copyright: © 2016, Springer-Verlag Berlin Heidelberg.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Anaerobic ammonium oxidation (anammox) and nitrite-dependent anaerobic methane oxidation (n-damo) are two new processes of recent discoveries linking the microbial nitrogen and carbon cycles. In this study, 16S ribosomal RNA (rRNA) gene of anammox bacteria and pmoA gene of n-damo bacteria were used to investigate their distribution and diversity in natural acidic and re-vegetated forest soils. The 16S rRNA gene sequences retrieved featured at least three species in two genera known anammox bacteria, namely Candidatus Brocadia anammoxidans, Candidatus Brocadia fulgida, and Candidatus Kuenenia stuttgartiensis while the pmoA gene amplified was affiliated with two species of known n-damo bacteria Candidatus Methylomirabilis oxyfera and a newly established Candidatus Methylomirabilis sp. According to the results, the diversity of anammox bacteria in natural forests was lower than in re-vegetated forests, but no significant difference was observed in n-damo community between them. Quantitative real-time PCR showed that both anammox and n-damo bacteria were more abundant in the lower layer (10–20 cm) than the surface layer (0–5 cm). The abundance of anammox bacteria varied from 2.21 × 105 to 3.90 × 106 gene copies per gram dry soil, and n-damo bacteria quantities were between 1.69 × 105 and 5.07 × 106 gene copies per gram dry soil in the two different layers. Both anammox and n-damo bacteria are reported for the first time to co-occur in acidic forest soil in this study, providing a more comprehensive information on more defined microbial processes contributing to C and N cycles in the ecosystems.

AB - Anaerobic ammonium oxidation (anammox) and nitrite-dependent anaerobic methane oxidation (n-damo) are two new processes of recent discoveries linking the microbial nitrogen and carbon cycles. In this study, 16S ribosomal RNA (rRNA) gene of anammox bacteria and pmoA gene of n-damo bacteria were used to investigate their distribution and diversity in natural acidic and re-vegetated forest soils. The 16S rRNA gene sequences retrieved featured at least three species in two genera known anammox bacteria, namely Candidatus Brocadia anammoxidans, Candidatus Brocadia fulgida, and Candidatus Kuenenia stuttgartiensis while the pmoA gene amplified was affiliated with two species of known n-damo bacteria Candidatus Methylomirabilis oxyfera and a newly established Candidatus Methylomirabilis sp. According to the results, the diversity of anammox bacteria in natural forests was lower than in re-vegetated forests, but no significant difference was observed in n-damo community between them. Quantitative real-time PCR showed that both anammox and n-damo bacteria were more abundant in the lower layer (10–20 cm) than the surface layer (0–5 cm). The abundance of anammox bacteria varied from 2.21 × 105 to 3.90 × 106 gene copies per gram dry soil, and n-damo bacteria quantities were between 1.69 × 105 and 5.07 × 106 gene copies per gram dry soil in the two different layers. Both anammox and n-damo bacteria are reported for the first time to co-occur in acidic forest soil in this study, providing a more comprehensive information on more defined microbial processes contributing to C and N cycles in the ecosystems.

KW - Anammox bacteria

KW - Carbon cycle

KW - Forest soil

KW - N-damo bacteria

KW - Nitrogen cycle

KW - pmoA gene

UR - http://www.scopus.com/inward/record.url?scp=84968608581&partnerID=8YFLogxK

U2 - 10.1007/s00253-016-7585-6

DO - 10.1007/s00253-016-7585-6

M3 - 文章

C2 - 27178181

AN - SCOPUS:84968608581

SN - 0175-7598

VL - 100

SP - 7727

EP - 7739

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 17

ER -