Chromate reduction by Bacillus megaterium TKW3 isolated from marine sediments

K. H. Cheung, Ji Dong Gu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Bacillus megaterium strain TKW3 was isolated from multiple-metal- contaminated marine sediments of Tokwawan, Hong Kong SAR. This facultative aerobe utilized arabinose, mannitol, N-acetylglucosamine, maltose, caprate, citrate, butyrate or lactate as the sole source of carbon and energy for growth.B. megaterium TKW3 reduced highly toxic and soluble Cr6+ (as CrO 4 2- ) into almost non-toxic and insoluble Cr 3+ under aerobic conditions. Complete reduction of 0.20 mM Cr 6+ by B. megaterium TKW3 was achieved within 360 h. Initial Cr 6+ concentration below 0.90 mM or inoculum less than 107 cells ml-1 did not have significant effect on 6+ reduction, while the residue Cr6+ concentration was the lowest at 107 cells ml-1. Cr6+ reduction by this strain was inhibited by high levels of NaCl (55%). B. megaterium TKW3 was also resistant to other oxyanions including 0.34 mM Cr2 O 7 2- 0.32 mM AsO 4 3- , 0.58 mM SeO 3 2- and 0.53 mM SeO 4 2- , and reduced soluble Se4+ (as SeO 3 2- ) to insoluble red amorphous Se0. B. megaterium TKW3 might have potential application in bioremediation of Cr-laden sediments associated with other oxyanions.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalWorld Journal of Microbiology and Biotechnology
Volume21
Issue number3
DOIs
StatePublished - Apr 2005
Externally publishedYes

Keywords

  • Bacillus megaterium
  • Bioremediation
  • Chromate reduction
  • Chromium
  • Metal resistance
  • Selenium

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