Characterization of two urease-producing and calcifying Bacillus spp. isolated from cement

Varenyam Achal*, Abhijit Mukherjee, M. Sudhakara Reddy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Two bacterial strains designated as CT2 and CT5 were isolated from highly alkaline cement samples using the enrichment culture technique. On the basis of various physiological tests and 16S rRNA sequence analysis, the bacteria were identified as Bacillus species. The urease production was 575.87 U/ml and 670.71 U/ml for CT2 and CT5, respectively. Calcite constituted 27.6% and 31% of the total weight of sand samples plugged by CT2 and CT5, respectively. Scanning electron micrography analysis revealed the direct involvement of these isolates in calcite precipitation. This is the first report of the isolation and identification of Bacillus species from cement. Based on the ability of these bacteria to tolerate the extreme environment of cement, they have potential to be used in remediating the cracks and fissures in various building or concrete structures.

Original languageEnglish
Pages (from-to)1571-1576
Number of pages6
JournalJournal of Microbiology and Biotechnology
Issue number11
StatePublished - Nov 2010
Externally publishedYes


  • 16S rRNA
  • Bacillus
  • Calcite precipitation
  • Cement
  • Crack remediation
  • Urease


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