Characterization of a Salmonella choleraesuis mutant that cannot multiply within epithelial cells

B. B. Finlay; S. Chatfield; K. Y. Leung; G. Dougan; S. Falkow

doi:10.1139/m91-095

Characterization of a Salmonella choleraesuis mutant that cannot multiply within epithelial cells

B. B. Finlay^*, S. Chatfield, K. Y. Leung, G. Dougan, S. Falkow

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A mutant of Salmonella choleraesuis was identified that could invade (enter) and penetrate through polarized monolayers of Caco-2 and MDCK epithelial cells at normal levels but was defective for intracellular multiplication within these cells. It was also able to survive inside cultured J774 macrophage cells. These bacteria remained inside membrane-bound vacuoles, which coalesced at later times in the perinuclear region of the epithelial cell. This mutant exhibited slightly slower growth rates in rich or minimal media than the parental strain but was normal for iron usage, phosphate usage, and anaerobic growth and was a prototroph. The mutant was completely avirulent when administered orally or intravenously to susceptible mice. These results suggest that the ability to multiply within eukaryotic cells may contribute to S. choleraesuis virulence.

Original language	English
Pages (from-to)	568-572
Number of pages	5
Journal	Canadian Journal of Microbiology
Volume	37
Issue number	7
DOIs	https://doi.org/10.1139/m91-095
State	Published - 1991
Externally published	Yes

Keywords

Intracellular Multiplication
Pathogenesis
Salmonella choleraesuis
Virulence

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title = "Characterization of a Salmonella choleraesuis mutant that cannot multiply within epithelial cells",

abstract = "A mutant of Salmonella choleraesuis was identified that could invade (enter) and penetrate through polarized monolayers of Caco-2 and MDCK epithelial cells at normal levels but was defective for intracellular multiplication within these cells. It was also able to survive inside cultured J774 macrophage cells. These bacteria remained inside membrane-bound vacuoles, which coalesced at later times in the perinuclear region of the epithelial cell. This mutant exhibited slightly slower growth rates in rich or minimal media than the parental strain but was normal for iron usage, phosphate usage, and anaerobic growth and was a prototroph. The mutant was completely avirulent when administered orally or intravenously to susceptible mice. These results suggest that the ability to multiply within eukaryotic cells may contribute to S. choleraesuis virulence.",

keywords = "Intracellular Multiplication, Pathogenesis, Salmonella choleraesuis, Virulence",

author = "Finlay, {B. B.} and S. Chatfield and Leung, {K. Y.} and G. Dougan and S. Falkow",

year = "1991",

doi = "10.1139/m91-095",

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journal = "Canadian Journal of Microbiology",

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publisher = "National Research Council of Canada",

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TY - JOUR

T1 - Characterization of a Salmonella choleraesuis mutant that cannot multiply within epithelial cells

AU - Finlay, B. B.

AU - Chatfield, S.

AU - Leung, K. Y.

AU - Dougan, G.

AU - Falkow, S.

PY - 1991

Y1 - 1991

N2 - A mutant of Salmonella choleraesuis was identified that could invade (enter) and penetrate through polarized monolayers of Caco-2 and MDCK epithelial cells at normal levels but was defective for intracellular multiplication within these cells. It was also able to survive inside cultured J774 macrophage cells. These bacteria remained inside membrane-bound vacuoles, which coalesced at later times in the perinuclear region of the epithelial cell. This mutant exhibited slightly slower growth rates in rich or minimal media than the parental strain but was normal for iron usage, phosphate usage, and anaerobic growth and was a prototroph. The mutant was completely avirulent when administered orally or intravenously to susceptible mice. These results suggest that the ability to multiply within eukaryotic cells may contribute to S. choleraesuis virulence.

AB - A mutant of Salmonella choleraesuis was identified that could invade (enter) and penetrate through polarized monolayers of Caco-2 and MDCK epithelial cells at normal levels but was defective for intracellular multiplication within these cells. It was also able to survive inside cultured J774 macrophage cells. These bacteria remained inside membrane-bound vacuoles, which coalesced at later times in the perinuclear region of the epithelial cell. This mutant exhibited slightly slower growth rates in rich or minimal media than the parental strain but was normal for iron usage, phosphate usage, and anaerobic growth and was a prototroph. The mutant was completely avirulent when administered orally or intravenously to susceptible mice. These results suggest that the ability to multiply within eukaryotic cells may contribute to S. choleraesuis virulence.

KW - Intracellular Multiplication

KW - Pathogenesis

KW - Salmonella choleraesuis

KW - Virulence

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DO - 10.1139/m91-095

M3 - 文章

C2 - 1913359

AN - SCOPUS:0025901030

SN - 0008-4166

VL - 37

SP - 568

EP - 572

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

IS - 7

ER -

Characterization of a Salmonella choleraesuis mutant that cannot multiply within epithelial cells

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Keywords

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