Carbon Sphere-Polyaniline Composite: A Fluorescent Scaffold for Proliferation of Adipose Derived Stem Cells and its Cellular uptake

TY - JOUR

T1 - Carbon Sphere-Polyaniline Composite

T2 - A Fluorescent Scaffold for Proliferation of Adipose Derived Stem Cells and its Cellular uptake

AU - Gandla, Dayakar

AU - Putta, Chandrababu

AU - Ghosh, Sutapa

AU - Hazra, Binoy Krishna

N1 - Publisher Copyright: © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2016/8/1

Y1 - 2016/8/1

N2 - In this work, for the first time, we used inherently fluorescent carbon spheres (IF-CSP) as a doping agent to retain the electroactivity of polyaniline (PANI) in neutral media by making composite (IF-CSP@PANI) which is an important requirement for physiological applications of PANI. To address this issue, in situ chemical oxidative polymerization of aniline is done in presence of IF-CSP. IF-CSP@PANI has higher electrochemical activity than PANI in cell culture media at pH 7.4 with high cycling stability which was confirmed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). In addition, this material showed great promise as a biocompatible scaffold for the adherence and proliferation of adipose derived stem cells (ADSCs) at all concentrations of IF-CSP@PANI composite (10-200 μg/mL) for five days of culture period and this was confirmed by MTT assay. Doping of PANI with IF-CSP might have helped to preserve its electroactivity and cycling stability at higher pH values by hindering the deprotonation of its conducting form. Proliferation of ADSCs may be attributed to the electroactivity of the composite material. Confocal laser scanning microscopy (CLSM) images evidenced that the IF-CSP@PANI composite was able to enter into the cytoplasm of ADSCs. These extraordinary properties might render the IF-CSP@PANI composite as a promising candidate for bioimaging, biosensing, tissue engineering and drug or gene delivery.

AB - In this work, for the first time, we used inherently fluorescent carbon spheres (IF-CSP) as a doping agent to retain the electroactivity of polyaniline (PANI) in neutral media by making composite (IF-CSP@PANI) which is an important requirement for physiological applications of PANI. To address this issue, in situ chemical oxidative polymerization of aniline is done in presence of IF-CSP. IF-CSP@PANI has higher electrochemical activity than PANI in cell culture media at pH 7.4 with high cycling stability which was confirmed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). In addition, this material showed great promise as a biocompatible scaffold for the adherence and proliferation of adipose derived stem cells (ADSCs) at all concentrations of IF-CSP@PANI composite (10-200 μg/mL) for five days of culture period and this was confirmed by MTT assay. Doping of PANI with IF-CSP might have helped to preserve its electroactivity and cycling stability at higher pH values by hindering the deprotonation of its conducting form. Proliferation of ADSCs may be attributed to the electroactivity of the composite material. Confocal laser scanning microscopy (CLSM) images evidenced that the IF-CSP@PANI composite was able to enter into the cytoplasm of ADSCs. These extraordinary properties might render the IF-CSP@PANI composite as a promising candidate for bioimaging, biosensing, tissue engineering and drug or gene delivery.

KW - Polyaniline

KW - cell culture media

KW - cellular uptake

KW - chemical oxidative polymerization

KW - electroactivity

KW - fluorescent carbon spheres

KW - tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85041943034&partnerID=8YFLogxK

U2 - 10.1002/slct.201600485

DO - 10.1002/slct.201600485

M3 - 文章

AN - SCOPUS:85041943034

SN - 2365-6549

VL - 1

SP - 3063

EP - 3070

JO - ChemistrySelect

JF - ChemistrySelect

IS - 12

ER -