Biotechnological Production of Flavonoids: An Update on Plant Metabolic Engineering, Microbial Host Selection, and Genetically Encoded Biosensors

Monireh Marsafari; Habibollah Samizadeh; Babak Rabiei; Ali Ashraf Mehrabi; Mattheos Koffas; Peng Xu

doi:10.1002/biot.201900432

Biotechnological Production of Flavonoids: An Update on Plant Metabolic Engineering, Microbial Host Selection, and Genetically Encoded Biosensors

Monireh Marsafari, Habibollah Samizadeh, Babak Rabiei, Ali Ashraf Mehrabi, Mattheos Koffas^*, Peng Xu^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

36 Scopus citations

Abstract

Flavonoids represent a diversified family of phenylpropanoid-derived plant secondary metabolites. They are widely found in fruits, vegetables, and medicinal herbs. There has been increasing interest on flavonoids because of their proven bioactivity associated with anti-obesity and anti-cancer, anti-inflammatory and anti-diabetic activity. Low bioavailability of flavonoids is a major challenge restricting their applications. Due to safety and economic issues, plant extraction or chemical synthesis could not provide a scalable route for large-scale production. Alternatively, reconstruction of biosynthetic gene clusters in plants and industrially relevant microbes offer significant promise for discovery and scalable synthesis of flavonoids. This review provides an update on biotechnological production of flavonoids. The recent advances on plant metabolic engineering, microbial host, and genetically encoded biosensors are summarized. Plant metabolic engineering holds the promise to improve the yield of specific flavonoids and expand the chemical space of novel flavonoids. The choice of microbial host provides the cellular chassis that could be tailored for various stereo- or regio-selective chemistries that are crucial for their bioactivities. When coupled with transcriptional biosensing, genetically encoded biosensors could be welded into cellular metabolism to achieve high throughput screening or dynamic carbon flux re-allocation to deliver efficient microbial workhorse. The convergence of these technologies will translate the vast majority of plant genetic resources into valuable flavonoids with pharmaceutical/nutraceutical values in the foreseeable future.

Original language	English
Article number	1900432
Journal	Biotechnology Journal
Volume	15
Issue number	8
DOIs	https://doi.org/10.1002/biot.201900432
State	Published - 1 Aug 2020
Externally published	Yes

Keywords

biosensor
flavonoids
microbial host
plant metabolic engineering
synthetic biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/biot.201900432

Cite this

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title = "Biotechnological Production of Flavonoids: An Update on Plant Metabolic Engineering, Microbial Host Selection, and Genetically Encoded Biosensors",

abstract = "Flavonoids represent a diversified family of phenylpropanoid-derived plant secondary metabolites. They are widely found in fruits, vegetables, and medicinal herbs. There has been increasing interest on flavonoids because of their proven bioactivity associated with anti-obesity and anti-cancer, anti-inflammatory and anti-diabetic activity. Low bioavailability of flavonoids is a major challenge restricting their applications. Due to safety and economic issues, plant extraction or chemical synthesis could not provide a scalable route for large-scale production. Alternatively, reconstruction of biosynthetic gene clusters in plants and industrially relevant microbes offer significant promise for discovery and scalable synthesis of flavonoids. This review provides an update on biotechnological production of flavonoids. The recent advances on plant metabolic engineering, microbial host, and genetically encoded biosensors are summarized. Plant metabolic engineering holds the promise to improve the yield of specific flavonoids and expand the chemical space of novel flavonoids. The choice of microbial host provides the cellular chassis that could be tailored for various stereo- or regio-selective chemistries that are crucial for their bioactivities. When coupled with transcriptional biosensing, genetically encoded biosensors could be welded into cellular metabolism to achieve high throughput screening or dynamic carbon flux re-allocation to deliver efficient microbial workhorse. The convergence of these technologies will translate the vast majority of plant genetic resources into valuable flavonoids with pharmaceutical/nutraceutical values in the foreseeable future.",

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author = "Monireh Marsafari and Habibollah Samizadeh and Babak Rabiei and Mehrabi, {Ali Ashraf} and Mattheos Koffas and Peng Xu",

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AU - Samizadeh, Habibollah

AU - Rabiei, Babak

AU - Mehrabi, Ali Ashraf

AU - Koffas, Mattheos

AU - Xu, Peng

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Biotechnological Production of Flavonoids: An Update on Plant Metabolic Engineering, Microbial Host Selection, and Genetically Encoded Biosensors

Abstract

Keywords

UN SDGs

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