Biodegradation of perchloroethylene and chlorophenol co-contamination and toxic effect on activated sludge performance

TY - JOUR

T1 - Biodegradation of perchloroethylene and chlorophenol co-contamination and toxic effect on activated sludge performance

AU - Li, Hui

AU - Shen, Ting Ting

AU - Wang, Xiao Li

AU - Lin, Kuang Fei

AU - Liu, Yong Di

AU - Lu, Shu Guang

AU - Gu, Ji Dong

AU - Wang, Ping

AU - Lu, Qiang

AU - Du, Xiao Ming

N1 - Funding Information: This research was financially supported by the National Natural Science Foundation of China ( 41003031 , 41273109 ), the Shanghai Rising-Star Program ( 12QA1400800 ), the Specialized Research Fund for the Doctoral Program of Higher Education ( 20110074130002 ), the National Environmental Protection Public Welfare Science and Technology Research Program of China ( 200909089 , 2010467032 , 201109013 ), the Key Science Foundation of Shanghai Environmental Protection Bureau (Huhuanke2012-07), the Fundamental Research Funds for the Central Universities (WB1014004,WB1314002), the National Science Foundation for Postdoctoral Scientists of China ( 2012M510811 ). We also would like to thank the anonymous referees for their helpful comments on this paper.

PY - 2013/6

Y1 - 2013/6

N2 - This study investigated the effects of PCE and 2-CP co-contamination on growth of microbial community in terms of enzymatic activity and microbial diversity in activated sludge. Results showed that the activities of three key enzymes (dehydrogenase, phosphatase and urease) decreased significantly when PCE (in the range of 5-150. mg/L) was mixed with 2-CP (in the range of 25-150. mg/L). Especially, activity of dehydrogenase decreased by more than 93% as the concentration of PCE and 2-CP both reached 150. mg/L. PCR-DGGE revealed that short-term exposure with PCE and 2-CP did not lead to shift in the microbial community structure, while clone library demonstrated a significant change in the microbial diversity after long-term exposure. As the population of Alphaproteobacteria and Gamaproteobacteria decreased, with Actinobacteria eventually disappeared, species including Firmicutes, Bacteroidetes and Synergistetes became dominating groups. This study demonstrated that co-contamination with PCE and 2-CP affected the performance of activated sludge in a significant way.

AB - This study investigated the effects of PCE and 2-CP co-contamination on growth of microbial community in terms of enzymatic activity and microbial diversity in activated sludge. Results showed that the activities of three key enzymes (dehydrogenase, phosphatase and urease) decreased significantly when PCE (in the range of 5-150. mg/L) was mixed with 2-CP (in the range of 25-150. mg/L). Especially, activity of dehydrogenase decreased by more than 93% as the concentration of PCE and 2-CP both reached 150. mg/L. PCR-DGGE revealed that short-term exposure with PCE and 2-CP did not lead to shift in the microbial community structure, while clone library demonstrated a significant change in the microbial diversity after long-term exposure. As the population of Alphaproteobacteria and Gamaproteobacteria decreased, with Actinobacteria eventually disappeared, species including Firmicutes, Bacteroidetes and Synergistetes became dominating groups. This study demonstrated that co-contamination with PCE and 2-CP affected the performance of activated sludge in a significant way.

KW - Activated sludge

KW - Co-contamination

KW - Enzyme activity

KW - Microbial community diversity

UR - http://www.scopus.com/inward/record.url?scp=84876303699&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2013.02.050

DO - 10.1016/j.biortech.2013.02.050

M3 - 文章

C2 - 23587831

AN - SCOPUS:84876303699

SN - 0960-8524

VL - 137

SP - 286

EP - 293

JO - Bioresource Technology

JF - Bioresource Technology

ER -