Biodegradation of benzene homologues in contaminated sediment of the East China Sea

TY - JOUR

T1 - Biodegradation of benzene homologues in contaminated sediment of the East China Sea

AU - Li, Hui

AU - Zhang, Qian

AU - Wang, Xiao Li

AU - Ma, Xing Yuan

AU - Lin, Kuang Fei

AU - Liu, Yong Di

AU - Gu, Ji Dong

AU - Lu, Shu Guang

AU - Shi, Lei

AU - Lu, Qiang

AU - Shen, Ting Ting

N1 - Funding Information: This research was financially supported by the National Natural Science Foundation of China ( 41003031 , 41273109 ), the Specialized Research Fund for the Doctoral Program of Higher Education ( 20110074130002 ), the Shanghai Rising-Star Program ( 12QA1400800 ), the National Environmental Protection Public Welfare Science and Technology Research Program of China ( 200909089 , 201109013 ), the Fundamental Research Funds for the Central Universities ( WB1014004 ), the National Science Foundation for Postdoctoral Scientists of China ( 2012M510811 ). We also would like to thank the anonymous referees for their helpful comments on this paper.

PY - 2012/11

Y1 - 2012/11

N2 - This study focused on acclimating a microbial enrichment to biodegrade benzene, toluene, ethylbenzene and xylenes (BTEX) in a wide range of salinity. The enrichment degraded 120. mg/L toluene within 5. d in the presence of 2. M NaCl or 150. mg/L toluene within 7. d in the presence of 1-1.5. M NaCl. PCR-DGGE (polymerase chain reaction-denatured gradient gel electrophoresis) profiles demonstrated the dominant species in the enrichments distributed between five main phyla: Gammaproteobacteria, Sphingobacteriia, Prolixibacter, Flavobacteriia and Firmicutes. The Marinobacter, Prolixibacter, Balneola, Zunongwangia, Halobacillus were the dominant genus. PCR detection of genotypes involved in bacterial BETX degradation revealed that the degradation pathways contained all the known initial oxidative attack of BTEX by monooxygenase and dioxygenase. And the subsequent ring fission was catalysed by catechol 1,2-dioxygenase and catechol 2,3-dioxygenase. Nuclear magnetic resonance (NMR) spectroscopy profiles showed that the bacterial consortium adjusted the osmotic pressure by ectoine and hydroxyectoine as compatible solutes to acclimate the different salinity conditions.

AB - This study focused on acclimating a microbial enrichment to biodegrade benzene, toluene, ethylbenzene and xylenes (BTEX) in a wide range of salinity. The enrichment degraded 120. mg/L toluene within 5. d in the presence of 2. M NaCl or 150. mg/L toluene within 7. d in the presence of 1-1.5. M NaCl. PCR-DGGE (polymerase chain reaction-denatured gradient gel electrophoresis) profiles demonstrated the dominant species in the enrichments distributed between five main phyla: Gammaproteobacteria, Sphingobacteriia, Prolixibacter, Flavobacteriia and Firmicutes. The Marinobacter, Prolixibacter, Balneola, Zunongwangia, Halobacillus were the dominant genus. PCR detection of genotypes involved in bacterial BETX degradation revealed that the degradation pathways contained all the known initial oxidative attack of BTEX by monooxygenase and dioxygenase. And the subsequent ring fission was catalysed by catechol 1,2-dioxygenase and catechol 2,3-dioxygenase. Nuclear magnetic resonance (NMR) spectroscopy profiles showed that the bacterial consortium adjusted the osmotic pressure by ectoine and hydroxyectoine as compatible solutes to acclimate the different salinity conditions.

KW - BTEX

KW - Bacterial community

KW - Biodegradation

KW - Compatible solutes

KW - Metabolic pathway

UR - http://www.scopus.com/inward/record.url?scp=84866173808&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2012.08.033

DO - 10.1016/j.biortech.2012.08.033

M3 - 文章

C2 - 22989641

AN - SCOPUS:84866173808

SN - 0960-8524

VL - 124

SP - 129

EP - 136

JO - Bioresource Technology

JF - Bioresource Technology

ER -