TY - JOUR
T1 - Biochemical characterization of different types of adherence of Vibrio species to fish epithelial cells
AU - Wang, X. H.
AU - Leung, K. Y.
PY - 2000/4
Y1 - 2000/4
N2 - Vibrio species are Gram-negative bacteria that cause a systemic infection in fish called vibriosis. The authors previously demonstrated that internalization cell interactions. Adherence is a prerequisite for successful internalization. In this study, the adherence capability of two invasive strains [V. anguillarum 811218-5W and G/Virus/5(3)] was compared with that of two non-invasive strains [V. damselae ATCC 33539 and V. anguillarum S2/5/93(2)] using adherence assays in three different types of fish cells (epithelial papillosum of carp, EPC; grunt-fin tissue, GF; and fat-head minnow epithelial cells, FHM). For all four strains there was no significant difference (P > 0.05) in the adherence to the different cell lines. V. anguillarum 811218-5W exhibited the highest adherence, followed by G/Virus/5(3) and S2/5/93(2); V. damselae ATCC 33539 showed the lowest adherence. The super-adherence characteristic of V. anguillarum 811218-5W on EPC cells was not affected by inhibitors, sugars, low temperature (4°C) incubation, or non-biological surfaces such as glass coverslips. The galactose-linked adherence characteristic of V. anguillarum G/Virus/5(3) to the EPC cells was partially inhibited by peptidase treatment of the fish cells, low-temperature incubation, and addition of sugars that contained galactose (such as lactose and N-acetyl-D-galactosamine). De novo synthesis of bacterial protein, viable bacteria and intact carbohydrate structure of vibrios were required for both super-adherence and galactose-linked adherence. These adherence characteristics were also found in ten other invasive vibrios, and galactose-linked adherence was found in nine invasive vibrios.
AB - Vibrio species are Gram-negative bacteria that cause a systemic infection in fish called vibriosis. The authors previously demonstrated that internalization cell interactions. Adherence is a prerequisite for successful internalization. In this study, the adherence capability of two invasive strains [V. anguillarum 811218-5W and G/Virus/5(3)] was compared with that of two non-invasive strains [V. damselae ATCC 33539 and V. anguillarum S2/5/93(2)] using adherence assays in three different types of fish cells (epithelial papillosum of carp, EPC; grunt-fin tissue, GF; and fat-head minnow epithelial cells, FHM). For all four strains there was no significant difference (P > 0.05) in the adherence to the different cell lines. V. anguillarum 811218-5W exhibited the highest adherence, followed by G/Virus/5(3) and S2/5/93(2); V. damselae ATCC 33539 showed the lowest adherence. The super-adherence characteristic of V. anguillarum 811218-5W on EPC cells was not affected by inhibitors, sugars, low temperature (4°C) incubation, or non-biological surfaces such as glass coverslips. The galactose-linked adherence characteristic of V. anguillarum G/Virus/5(3) to the EPC cells was partially inhibited by peptidase treatment of the fish cells, low-temperature incubation, and addition of sugars that contained galactose (such as lactose and N-acetyl-D-galactosamine). De novo synthesis of bacterial protein, viable bacteria and intact carbohydrate structure of vibrios were required for both super-adherence and galactose-linked adherence. These adherence characteristics were also found in ten other invasive vibrios, and galactose-linked adherence was found in nine invasive vibrios.
KW - Adherence
KW - Fish epithelial cells
KW - Vibrio spp.
UR - http://www.scopus.com/inward/record.url?scp=0034077892&partnerID=8YFLogxK
U2 - 10.1099/00221287-146-4-989
DO - 10.1099/00221287-146-4-989
M3 - 文章
C2 - 10784057
AN - SCOPUS:0034077892
VL - 146
SP - 989
EP - 998
JO - Microbiology (United Kingdom)
JF - Microbiology (United Kingdom)
SN - 1350-0872
IS - 4
ER -