Assessment of Five Electron-Shuttling Molecules in the Extracellular Electron Transfer of Electromethanogenesis by using Methanosarcina barkeri

Tian Tian Liang, Lei Zhou, Muhammad Irfan, Yang Bai, Xue Zhi Liu, Ji Liang Zhang, Zong Yang Wu, Wen Zhuo Wang, Jin Feng Liu, Lei Cheng, Shi Zhong Yang, Ru Qiang Ye, Ji Dong Gu, Bo Zhong Mu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Electron-shuttling molecules (ESMs), natural or synthetic, are utilized by microorganisms as electron acceptors or donors to facilitate electron transfer. In this study, five ESMs, namely anthraquinone-2-carboxylic acid (AQC), 9,10-anthraquinone-2-sulfonicacid (AQS), 9,10-anthraquinone-2,6-disulfonic acid (AQDS), Neutral red, and Thionin, were selected to assess their effects on electromethanogenesis under different electrochemical potentials and concentrations. Results showed that the rate of methanogenesis achieved in the presence of AQC was 7.4 times higher than that of the control (without ESMs) at −850 mV (vs. Ag/AgCl). The effectiveness on methanogenesis followed AQC, Neutral Red, AQS, AQDS, and Thionin in a decreasing order. Compared with the control, the addition of ESMs did not affect the biomass of methanogens significantly. Meanwhile, qPCR analysis of the ccdA gene indicated that the abundance of the Cytochrome c gene and the extent of CO2 reduction to methane correlated positively. The promoting effect of the selective ESMs on methanogenesis was mainly related to their electron transfer capabilities.

Original languageEnglish
Pages (from-to)3783-3789
Number of pages7
JournalChemElectroChem
Volume7
Issue number18
DOIs
StatePublished - 15 Sep 2020

Keywords

  • AQC
  • CO
  • CO storage
  • ccdA gene
  • electromethanogenesis
  • electron shuttling molecules

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