Application of scanning fluorometry for monitoring of a fermentation process

B. Tartakovsky; M. Sheintuch; J. M. Hilmer; T. Scheper

doi:10.1021/bp950045h

Application of scanning fluorometry for monitoring of a fermentation process

B. Tartakovsky, M. Sheintuch^*, J. M. Hilmer, T. Scheper

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

Conventional observation of a fermentation process by using NAD(P)H-dependent fluorescence provides a mean for cell monitoring, but its practical application has been very limited due to many factors that affect culture fluorescence. In this work we studied correlation of scanning fluorometry signals with various process variables and with cell metabolic states and showed that scanning fluorometry is more informative than conventional NAD(P)H-dependent fluorescence. A stepwise multiple-regression procedure has been used to select meaningful signals from the whole spectrum, and we found that with three or four components of the excitation-emission plane the process variables can be portrayed with a rather good accuracy. The approach is demonstrated on the examples of wild-type or recombinant Escherichia coli and Saccharomyces cerevisiae fermentations.

Original language	English
Pages (from-to)	126-131
Number of pages	6
Journal	Biotechnology Progress
Volume	12
Issue number	1
DOIs	https://doi.org/10.1021/bp950045h
State	Published - 1996
Externally published	Yes

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abstract = "Conventional observation of a fermentation process by using NAD(P)H-dependent fluorescence provides a mean for cell monitoring, but its practical application has been very limited due to many factors that affect culture fluorescence. In this work we studied correlation of scanning fluorometry signals with various process variables and with cell metabolic states and showed that scanning fluorometry is more informative than conventional NAD(P)H-dependent fluorescence. A stepwise multiple-regression procedure has been used to select meaningful signals from the whole spectrum, and we found that with three or four components of the excitation-emission plane the process variables can be portrayed with a rather good accuracy. The approach is demonstrated on the examples of wild-type or recombinant Escherichia coli and Saccharomyces cerevisiae fermentations.",

author = "B. Tartakovsky and M. Sheintuch and Hilmer, {J. M.} and T. Scheper",

year = "1996",

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T1 - Application of scanning fluorometry for monitoring of a fermentation process

AU - Tartakovsky, B.

AU - Sheintuch, M.

AU - Hilmer, J. M.

AU - Scheper, T.

PY - 1996

Y1 - 1996

N2 - Conventional observation of a fermentation process by using NAD(P)H-dependent fluorescence provides a mean for cell monitoring, but its practical application has been very limited due to many factors that affect culture fluorescence. In this work we studied correlation of scanning fluorometry signals with various process variables and with cell metabolic states and showed that scanning fluorometry is more informative than conventional NAD(P)H-dependent fluorescence. A stepwise multiple-regression procedure has been used to select meaningful signals from the whole spectrum, and we found that with three or four components of the excitation-emission plane the process variables can be portrayed with a rather good accuracy. The approach is demonstrated on the examples of wild-type or recombinant Escherichia coli and Saccharomyces cerevisiae fermentations.

AB - Conventional observation of a fermentation process by using NAD(P)H-dependent fluorescence provides a mean for cell monitoring, but its practical application has been very limited due to many factors that affect culture fluorescence. In this work we studied correlation of scanning fluorometry signals with various process variables and with cell metabolic states and showed that scanning fluorometry is more informative than conventional NAD(P)H-dependent fluorescence. A stepwise multiple-regression procedure has been used to select meaningful signals from the whole spectrum, and we found that with three or four components of the excitation-emission plane the process variables can be portrayed with a rather good accuracy. The approach is demonstrated on the examples of wild-type or recombinant Escherichia coli and Saccharomyces cerevisiae fermentations.

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DO - 10.1021/bp950045h

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SN - 8756-7938

VL - 12

SP - 126

EP - 131

JO - Biotechnology Progress

JF - Biotechnology Progress

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Application of scanning fluorometry for monitoring of a fermentation process

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