Application of scanning fluorometry for monitoring of a fermentation process

B. Tartakovsky, M. Sheintuch*, J. M. Hilmer, T. Scheper

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Conventional observation of a fermentation process by using NAD(P)H-dependent fluorescence provides a mean for cell monitoring, but its practical application has been very limited due to many factors that affect culture fluorescence. In this work we studied correlation of scanning fluorometry signals with various process variables and with cell metabolic states and showed that scanning fluorometry is more informative than conventional NAD(P)H-dependent fluorescence. A stepwise multiple-regression procedure has been used to select meaningful signals from the whole spectrum, and we found that with three or four components of the excitation-emission plane the process variables can be portrayed with a rather good accuracy. The approach is demonstrated on the examples of wild-type or recombinant Escherichia coli and Saccharomyces cerevisiae fermentations.

Original languageEnglish
Pages (from-to)126-131
Number of pages6
JournalBiotechnology Progress
Volume12
Issue number1
DOIs
StatePublished - 1996
Externally publishedYes

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