Analysis of parental strain DNA fragments existing in GEMs-Fhhh

Chun bo Hao, Jun Yan, Meng meng Qu, Dong Wang, Shu pei Cheng*, Ji dong Gu, Wan fei Qiu, Yin yin Wang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

There were 6 target DNA fragments of the three parental strains existing in the cell of GEMs(genetically engineered microorganism strain) Fhhh measured in this research by PCR(polymerase chain reaction). The determination showed that GEMs Fhhh contained all the 6 target DNA fragments, mnp1, mnp2, lip1, lip2, FLO1 and 16S rDNA, and had the molecular genetic stability. Meanwhile the PCR production of each parental strain could only had its target DNA fragments and was different from each other. It may illustrate that the technique of the inter-kingdom protoplast fusion for the construction of GEMs Fhhh through the process of intercellular gene recombination could be used as a reliable bioengineering technique to create the specific functional stain for the pollution control.

Original languageEnglish
Pages (from-to)590-594
Number of pages5
JournalJournal of Environmental Sciences
Volume15
Issue number5
StatePublished - Sep 2003
Externally publishedYes

Keywords

  • DNA fragment
  • GEMs
  • PCR
  • Pollution control
  • Protoplast fusion
  • Target gene

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