TY - JOUR
T1 - An internal recycling mechanism between ammonia/ammonium and nitrate driven by ammonia-oxidizing archaea and bacteria (AOA, AOB, and Comammox) and DNRA on Angkor sandstone monuments
AU - Ding, Xinghua
AU - Lan, Wensheng
AU - Li, Yiliang
AU - Yan, Aixin
AU - Katayama, Yoko
AU - Koba, Keisuke
AU - Makabe, Akiko
AU - Fukushima, Keitaro
AU - Yano, Midori
AU - Onishi, Yuji
AU - Ge, Qinya
AU - Gu, Jidong
PY - 2021/11/1
Y1 - 2021/11/1
N2 - The ancient Angkorian sandstone monuments in Cambodia are well known for biodeterioration of the sandstone under tropic climate. This study examined ammonia oxidizing bacteria (AOB and Comammox) and archaea (AOA) in stone-dwelling microbiome from three important Angkor monuments, namely Angor Wat, Bayon of Angkor Thom, and Preah Vihear, by DNA-based metagenomics analysis, RNA-based functional gene reverse-transcriptional (RT)-qPCR quantification, and N-15 isotope analysis. Our metagenomics datasets on these three monuments reveal a rich microbiome and abundant microbial nitrogen transforming reactions of ammonia oxidation and nitrite oxidation, Comammox and dissimilatory nitrate reduction to ammonium (DNRA), and these results support an internal recycling mechanism between ammonia/ammonium and nitrate by AOA, AOB, Comammox and DNRA on the stone monuments to support the microbial community. Though a wide range of AOB and AOA lineages together with Comammox were retrieved from these metagenomes as the potential ammonia-oxidizers, only AOA and Comammox were most abundant, likely to contribute to the biochemical processes of ammonia oxidation. The RNA-based qPCR quantification of the functional gene amoA in this study showed that AOA were the more active ammonia oxidizers over AOB in the microbiome of these Angkor sandstone monuments. In addition, a rich population of nitrite/nitrate producing AOA and Comammox drove the sequestration of CO2 onto the stone and this process was in turn supported by DNRA to provide the substrate ammonia/ammonium for a further cyclic reaction to take place continuously between ammonia/ammonium and nitrate. This model is further supported by the high enrichment of stable isotope 15N signature of NO3− in sandstone surface biofilms. The findings of this study are insightful for elucidation of nitrate accumulation by an internal N cycling mechanism proposed, and are important for understanding the sustainable microbial community and protection management of stone cultural heritage.
AB - The ancient Angkorian sandstone monuments in Cambodia are well known for biodeterioration of the sandstone under tropic climate. This study examined ammonia oxidizing bacteria (AOB and Comammox) and archaea (AOA) in stone-dwelling microbiome from three important Angkor monuments, namely Angor Wat, Bayon of Angkor Thom, and Preah Vihear, by DNA-based metagenomics analysis, RNA-based functional gene reverse-transcriptional (RT)-qPCR quantification, and N-15 isotope analysis. Our metagenomics datasets on these three monuments reveal a rich microbiome and abundant microbial nitrogen transforming reactions of ammonia oxidation and nitrite oxidation, Comammox and dissimilatory nitrate reduction to ammonium (DNRA), and these results support an internal recycling mechanism between ammonia/ammonium and nitrate by AOA, AOB, Comammox and DNRA on the stone monuments to support the microbial community. Though a wide range of AOB and AOA lineages together with Comammox were retrieved from these metagenomes as the potential ammonia-oxidizers, only AOA and Comammox were most abundant, likely to contribute to the biochemical processes of ammonia oxidation. The RNA-based qPCR quantification of the functional gene amoA in this study showed that AOA were the more active ammonia oxidizers over AOB in the microbiome of these Angkor sandstone monuments. In addition, a rich population of nitrite/nitrate producing AOA and Comammox drove the sequestration of CO2 onto the stone and this process was in turn supported by DNRA to provide the substrate ammonia/ammonium for a further cyclic reaction to take place continuously between ammonia/ammonium and nitrate. This model is further supported by the high enrichment of stable isotope 15N signature of NO3− in sandstone surface biofilms. The findings of this study are insightful for elucidation of nitrate accumulation by an internal N cycling mechanism proposed, and are important for understanding the sustainable microbial community and protection management of stone cultural heritage.
KW - Ammonia oxidizing archaea
KW - Ammonia oxidizing bacteria
KW - Biodeterioration
KW - Comammox
KW - Dissimilatory nitrate reduction to ammonia
KW - Nitrogen cycle
KW - Stone
UR - https://www.mendeley.com/catalogue/41ff51cb-237b-3940-9740-247aaab107ad/
U2 - 10.1016/j.ibiod.2021.105328
DO - 10.1016/j.ibiod.2021.105328
M3 - Article
SN - 0964-8305
VL - 165
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
ER -