AFLP and RFLP linkage map in Coix

Feng Qin, Jiansheng Li*, Xinhai Li, Harold Corke

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Coix is a genus in the grass family placed in the tribe Maydeae. It is closely related to maize and is also used as a crop plant. Since many valuable traits have been identified recently in Coix, it is considered to be a valuable genetic resource, particularly for maize improvement. In this study, a Coix genetic linkage map was constructed using an F2 population of 131 individuals. Eighty AFLP and 10 RFLP markers were mapped, covering a total length of 1339.5 cM with an average interval of 14.88 cM. The map consisted of 10 linkage groups, were consistent with the chromosome numbers observed cytogenetically. Both AFLP and RFLP markers were used first for genetic analysis in Coix. AFLP markers were generated by two restriction enzyme combinations, EcoRI/MseI and PstI/MseI. A total of 1349 bands were amplified, of which 140 were polymorphic. The polymorphism detection efficiency of the two enzyme combinations was compared, and utility of AFLP markers to construct the linkage map was discussed. Ten RFLP markers detected by three different probes were distributed on eight different linkage groups. The results provide a foundation to map and isolate important genes in Coix, and to investigate its genomic architecture, possible origins, and relationship with maize at the DNA level.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalGenetic Resources and Crop Evolution
Volume52
Issue number2
DOIs
StatePublished - Mar 2005
Externally publishedYes

Keywords

  • AFLP
  • Coix
  • Genetic map
  • RFLP

Fingerprint Dive into the research topics of 'AFLP and RFLP linkage map in Coix'. Together they form a unique fingerprint.

Cite this