TY - JOUR
T1 - AFLP and RFLP linkage map in Coix
AU - Qin, Feng
AU - Li, Jiansheng
AU - Li, Xinhai
AU - Corke, Harold
N1 - Funding Information:
The authors are grateful to the maize RFLP lab at University of Missouri, Columbia, for providing maize probes and also to Cornell University for rice and barley probes. This project was partially supported by IFS, NNSF of China (30170582), and a CRCG award from the University of Hong Kong.
PY - 2005/3
Y1 - 2005/3
N2 - Coix is a genus in the grass family placed in the tribe Maydeae. It is closely related to maize and is also used as a crop plant. Since many valuable traits have been identified recently in Coix, it is considered to be a valuable genetic resource, particularly for maize improvement. In this study, a Coix genetic linkage map was constructed using an F2 population of 131 individuals. Eighty AFLP and 10 RFLP markers were mapped, covering a total length of 1339.5 cM with an average interval of 14.88 cM. The map consisted of 10 linkage groups, were consistent with the chromosome numbers observed cytogenetically. Both AFLP and RFLP markers were used first for genetic analysis in Coix. AFLP markers were generated by two restriction enzyme combinations, EcoRI/MseI and PstI/MseI. A total of 1349 bands were amplified, of which 140 were polymorphic. The polymorphism detection efficiency of the two enzyme combinations was compared, and utility of AFLP markers to construct the linkage map was discussed. Ten RFLP markers detected by three different probes were distributed on eight different linkage groups. The results provide a foundation to map and isolate important genes in Coix, and to investigate its genomic architecture, possible origins, and relationship with maize at the DNA level.
AB - Coix is a genus in the grass family placed in the tribe Maydeae. It is closely related to maize and is also used as a crop plant. Since many valuable traits have been identified recently in Coix, it is considered to be a valuable genetic resource, particularly for maize improvement. In this study, a Coix genetic linkage map was constructed using an F2 population of 131 individuals. Eighty AFLP and 10 RFLP markers were mapped, covering a total length of 1339.5 cM with an average interval of 14.88 cM. The map consisted of 10 linkage groups, were consistent with the chromosome numbers observed cytogenetically. Both AFLP and RFLP markers were used first for genetic analysis in Coix. AFLP markers were generated by two restriction enzyme combinations, EcoRI/MseI and PstI/MseI. A total of 1349 bands were amplified, of which 140 were polymorphic. The polymorphism detection efficiency of the two enzyme combinations was compared, and utility of AFLP markers to construct the linkage map was discussed. Ten RFLP markers detected by three different probes were distributed on eight different linkage groups. The results provide a foundation to map and isolate important genes in Coix, and to investigate its genomic architecture, possible origins, and relationship with maize at the DNA level.
KW - AFLP
KW - Coix
KW - Genetic map
KW - RFLP
UR - http://www.scopus.com/inward/record.url?scp=17744384775&partnerID=8YFLogxK
U2 - 10.1007/s10722-003-1387-2
DO - 10.1007/s10722-003-1387-2
M3 - 文章
AN - SCOPUS:17744384775
SN - 0925-9864
VL - 52
SP - 209
EP - 214
JO - Genetic Resources and Crop Evolution
JF - Genetic Resources and Crop Evolution
IS - 2
ER -