Advanced genome-editing technologies enable rapid and large-scale generation of genetic variants for strain engineering and synthetic biology

Yi Zeng; Yuxiang Hong; Fidelis Azi; Yugeng Liu; Yousheng Chen; Chuchu Guo; Dewei Lin; Zizhao Wu; Wenhao Chen; Peng Xu

doi:10.1016/j.mib.2022.102175

Advanced genome-editing technologies enable rapid and large-scale generation of genetic variants for strain engineering and synthetic biology

Yi Zeng, Yuxiang Hong, Fidelis Azi, Yugeng Liu, Yousheng Chen, Chuchu Guo, Dewei Lin, Zizhao Wu, Wenhao Chen, Peng Xu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Targeted genome editing not only improves our understanding of fundamental rules in life sciences but also affords us versatile toolkits to improve industrially relevant phenotypes in various host cells. In this review, we summarize the recent endeavor to develop efficient genome-editing tools, and emphasize the utility of these tools to generate massive scale of genetic variants. We categorize these tools into traditional recombination-based tools, and more advanced CRISPR as well as RNA-based genome-editing tools. This diverse panel of sophisticated tools has been applied to accelerate strain engineering, upgrade biomanufacturing, and customize biosensing. In parallel with high-throughput phenotyping and AI-based optimization algorithms, we envision that genome-editing technologies will become a driving force to automate and streamline biological engineering, and empower us to address critical challenges in health, environment, energy, and sustainability.

Original language	English
Journal	Current Opinion in Microbiology
Volume	69
DOIs	https://doi.org/10.1016/j.mib.2022.102175
State	Published - 1 Oct 2022

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title = "Advanced genome-editing technologies enable rapid and large-scale generation of genetic variants for strain engineering and synthetic biology",

abstract = "Targeted genome editing not only improves our understanding of fundamental rules in life sciences but also affords us versatile toolkits to improve industrially relevant phenotypes in various host cells. In this review, we summarize the recent endeavor to develop efficient genome-editing tools, and emphasize the utility of these tools to generate massive scale of genetic variants. We categorize these tools into traditional recombination-based tools, and more advanced CRISPR as well as RNA-based genome-editing tools. This diverse panel of sophisticated tools has been applied to accelerate strain engineering, upgrade biomanufacturing, and customize biosensing. In parallel with high-throughput phenotyping and AI-based optimization algorithms, we envision that genome-editing technologies will become a driving force to automate and streamline biological engineering, and empower us to address critical challenges in health, environment, energy, and sustainability.",

author = "Yi Zeng and Yuxiang Hong and Fidelis Azi and Yugeng Liu and Yousheng Chen and Chuchu Guo and Dewei Lin and Zizhao Wu and Wenhao Chen and Peng Xu",

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AU - Zeng, Yi

AU - Hong, Yuxiang

AU - Azi, Fidelis

AU - Liu, Yugeng

AU - Chen, Yousheng

AU - Guo, Chuchu

AU - Lin, Dewei

AU - Wu, Zizhao

AU - Chen, Wenhao

AU - Xu, Peng

PY - 2022/10/1

Y1 - 2022/10/1

N2 - Targeted genome editing not only improves our understanding of fundamental rules in life sciences but also affords us versatile toolkits to improve industrially relevant phenotypes in various host cells. In this review, we summarize the recent endeavor to develop efficient genome-editing tools, and emphasize the utility of these tools to generate massive scale of genetic variants. We categorize these tools into traditional recombination-based tools, and more advanced CRISPR as well as RNA-based genome-editing tools. This diverse panel of sophisticated tools has been applied to accelerate strain engineering, upgrade biomanufacturing, and customize biosensing. In parallel with high-throughput phenotyping and AI-based optimization algorithms, we envision that genome-editing technologies will become a driving force to automate and streamline biological engineering, and empower us to address critical challenges in health, environment, energy, and sustainability.

AB - Targeted genome editing not only improves our understanding of fundamental rules in life sciences but also affords us versatile toolkits to improve industrially relevant phenotypes in various host cells. In this review, we summarize the recent endeavor to develop efficient genome-editing tools, and emphasize the utility of these tools to generate massive scale of genetic variants. We categorize these tools into traditional recombination-based tools, and more advanced CRISPR as well as RNA-based genome-editing tools. This diverse panel of sophisticated tools has been applied to accelerate strain engineering, upgrade biomanufacturing, and customize biosensing. In parallel with high-throughput phenotyping and AI-based optimization algorithms, we envision that genome-editing technologies will become a driving force to automate and streamline biological engineering, and empower us to address critical challenges in health, environment, energy, and sustainability.

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DO - 10.1016/j.mib.2022.102175

M3 - 文章

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JO - Current Opinion in Microbiology

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Advanced genome-editing technologies enable rapid and large-scale generation of genetic variants for strain engineering and synthetic biology

Abstract

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