A novel bacterium involved in the degradation of 2-methylindole isolated from sediment of inner deep bay of hong kong

TY - JOUR

T1 - A novel bacterium involved in the degradation of 2-methylindole isolated from sediment of inner deep bay of hong kong

AU - Yip, Karen Choi Wan

AU - Gu, Ji Dong

N1 - Publisher Copyright: © 2016 Karen Choi-Wan Yip and Ji-Dong Gu.

PY - 2016

Y1 - 2016

N2 - A bacterial strain, designated as MPKc, was isolated from the mudflat sediment of Mai Po Inner Deep Bay of Hong Kong Mai Po Nature Reserve by enrichment culturing with 2-methylindole as the sole source of carbon and energy. The microorganism was a Gram-negative, rod-shaped (0.4–0.6 μm × 1.0–2.2 μm) and aerobic bacterium. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain MPKc should be assigned as a novel bacterium, at least, at the species level. The 16S rDNA sequence most similiar to that of strain MPKc was Azoarcus evansii (94%) from available 16S rDNA sequences of the GenBank, indicating that strain MPKc was a member of the β-subclass of the Proteobacteria. Biochemical tests showed that strain MPKc was able to reduce nitrate to nitrogen. Carbon sources utilized by this strain included adipic acid, malate, citrate and phenylacetic acid although it only grew weakly on glucose, arabinose, mannose, mannitol, N-acetyl-glucosamine, maltose and gluconate. Strain MPKc showed no growth on capric acid. Its optimal growth occurred at 30°C, pH 6.5–7.5 and salinity 5–10‰. Strain MPKc was capable of degrading 80 μM 2-methylindole in 7 days under aerobic conditions. The possible chemical pathway for 2-methylindole degradation is through oxidation at 3-position or/and 2-position of the pyrrole ring.

AB - A bacterial strain, designated as MPKc, was isolated from the mudflat sediment of Mai Po Inner Deep Bay of Hong Kong Mai Po Nature Reserve by enrichment culturing with 2-methylindole as the sole source of carbon and energy. The microorganism was a Gram-negative, rod-shaped (0.4–0.6 μm × 1.0–2.2 μm) and aerobic bacterium. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain MPKc should be assigned as a novel bacterium, at least, at the species level. The 16S rDNA sequence most similiar to that of strain MPKc was Azoarcus evansii (94%) from available 16S rDNA sequences of the GenBank, indicating that strain MPKc was a member of the β-subclass of the Proteobacteria. Biochemical tests showed that strain MPKc was able to reduce nitrate to nitrogen. Carbon sources utilized by this strain included adipic acid, malate, citrate and phenylacetic acid although it only grew weakly on glucose, arabinose, mannose, mannitol, N-acetyl-glucosamine, maltose and gluconate. Strain MPKc showed no growth on capric acid. Its optimal growth occurred at 30°C, pH 6.5–7.5 and salinity 5–10‰. Strain MPKc was capable of degrading 80 μM 2-methylindole in 7 days under aerobic conditions. The possible chemical pathway for 2-methylindole degradation is through oxidation at 3-position or/and 2-position of the pyrrole ring.

KW - 3-methylindole

KW - Culturability

KW - Mai Po Nature Reserve

KW - Metabolism

UR - http://www.scopus.com/inward/record.url?scp=85045469779&partnerID=8YFLogxK

U2 - 10.26789/AEB.2016.01.008

DO - 10.26789/AEB.2016.01.008

M3 - 文章

AN - SCOPUS:85045469779

SN - 2382-6436

VL - 1

SP - 52

EP - 63

JO - Applied Environmental Biotechnology

JF - Applied Environmental Biotechnology

IS - 1

ER -