A comparative study of microstructural development in paired human hepatic and gallbladder biles

Daphne Weihs; Judith Schmidt; Dganit Danino; Ilana Goldiner; Diana Leikin-Gobbi; Arieh Eitan; Moshe Rubin; Yeshayahu Talmon; Fred M. Konikoff

doi:10.1016/j.bbalip.2007.07.006

A comparative study of microstructural development in paired human hepatic and gallbladder biles

Daphne Weihs^*, Judith Schmidt, Dganit Danino, Ilana Goldiner, Diana Leikin-Gobbi, Arieh Eitan, Moshe Rubin, Yeshayahu Talmon, Fred M. Konikoff

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Cholesterol gallstones usually develop in the gallbladder and rarely form in bile ducts even in patients with highly lithogenic bile. Bile concentration and proteins (e.g. mucin) may affect crystallization, but the exact nature of this effect, especially in relation to crystallization pathways and microstructural evolution remains unclear. We examined lipid microstructures in paired hepatic and gallbladder biles to reveal ones that are essential for crystallization. Combining digital light microscopy with cryogenic-temperature transmission electron microscopy we are able to directly visualize and compare the time evolution of lipid microstructures in paired hepatic, gallbladder and diluted gallbladder biles of gallstone patients and controls, without drying or separating. Gallbladder bile exhibited several multilamellar vesicles and spheroidal micelles preceding and throughout crystallization. Vesicle morphology changed before crystallization was observed. In contrast, hepatic bile revealed almost no crystallization and while a variety of unilamellar vesicles and spheroidal micelles existed throughout the examination, multilamellar vesicles were rare. Diluted gallbladder bile was different from native gallbladder bile, as well as the paired hepatic bile, yielding occasional crystallization. Our findings suggest that maturing multilamellar vesicles precede (and at least partially initiate) crystallization in gallbladder bile. Although microstructural development seems to be concentration dependent, dilution of gallbladder bile to hepatic bile concentrations neither makes it identical to hepatic bile, nor prevents crystallization.

Original language	English
Pages (from-to)	1289-1298
Number of pages	10
Journal	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume	1771
Issue number	10
DOIs	https://doi.org/10.1016/j.bbalip.2007.07.006
State	Published - Oct 2007
Externally published	Yes

Keywords

Cholesterol gallstones
Cryogenic-temperature transmission electron microscopy
Digital light microscopy
Lipid microstructures

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10.1016/j.bbalip.2007.07.006

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Weihs, Daphne ; Schmidt, Judith ; Danino, Dganit ; Goldiner, Ilana ; Leikin-Gobbi, Diana ; Eitan, Arieh ; Rubin, Moshe ; Talmon, Yeshayahu ; Konikoff, Fred M. / A comparative study of microstructural development in paired human hepatic and gallbladder biles. In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2007 ; Vol. 1771, No. 10. pp. 1289-1298.

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title = "A comparative study of microstructural development in paired human hepatic and gallbladder biles",

abstract = "Cholesterol gallstones usually develop in the gallbladder and rarely form in bile ducts even in patients with highly lithogenic bile. Bile concentration and proteins (e.g. mucin) may affect crystallization, but the exact nature of this effect, especially in relation to crystallization pathways and microstructural evolution remains unclear. We examined lipid microstructures in paired hepatic and gallbladder biles to reveal ones that are essential for crystallization. Combining digital light microscopy with cryogenic-temperature transmission electron microscopy we are able to directly visualize and compare the time evolution of lipid microstructures in paired hepatic, gallbladder and diluted gallbladder biles of gallstone patients and controls, without drying or separating. Gallbladder bile exhibited several multilamellar vesicles and spheroidal micelles preceding and throughout crystallization. Vesicle morphology changed before crystallization was observed. In contrast, hepatic bile revealed almost no crystallization and while a variety of unilamellar vesicles and spheroidal micelles existed throughout the examination, multilamellar vesicles were rare. Diluted gallbladder bile was different from native gallbladder bile, as well as the paired hepatic bile, yielding occasional crystallization. Our findings suggest that maturing multilamellar vesicles precede (and at least partially initiate) crystallization in gallbladder bile. Although microstructural development seems to be concentration dependent, dilution of gallbladder bile to hepatic bile concentrations neither makes it identical to hepatic bile, nor prevents crystallization.",

keywords = "Cholesterol gallstones, Cryogenic-temperature transmission electron microscopy, Digital light microscopy, Lipid microstructures",

author = "Daphne Weihs and Judith Schmidt and Dganit Danino and Ilana Goldiner and Diana Leikin-Gobbi and Arieh Eitan and Moshe Rubin and Yeshayahu Talmon and Konikoff, {Fred M.}",

note = "Funding Information: We thank Mrs. B. Shdemati for her assistance in sample preparation. DW is grateful for the Levi Eshkol Scholarship awarded to her through the Israeli Ministry of Science and Technology. The work was done with partial financial support of the Israeli Ministry of Health. Microscopy was performed at the Hannah and George Krumholz Laboratory for Advanced Microscopy at the Technion, part of the Technion Project on Complex Fluids.",

year = "2007",

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doi = "10.1016/j.bbalip.2007.07.006",

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journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",

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A comparative study of microstructural development in paired human hepatic and gallbladder biles. / Weihs, Daphne; Schmidt, Judith; Danino, Dganit; Goldiner, Ilana; Leikin-Gobbi, Diana; Eitan, Arieh; Rubin, Moshe; Talmon, Yeshayahu; Konikoff, Fred M.

In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1771, No. 10, 10.2007, p. 1289-1298.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A comparative study of microstructural development in paired human hepatic and gallbladder biles

AU - Weihs, Daphne

AU - Schmidt, Judith

AU - Danino, Dganit

AU - Goldiner, Ilana

AU - Leikin-Gobbi, Diana

AU - Eitan, Arieh

AU - Rubin, Moshe

AU - Talmon, Yeshayahu

AU - Konikoff, Fred M.

N1 - Funding Information: We thank Mrs. B. Shdemati for her assistance in sample preparation. DW is grateful for the Levi Eshkol Scholarship awarded to her through the Israeli Ministry of Science and Technology. The work was done with partial financial support of the Israeli Ministry of Health. Microscopy was performed at the Hannah and George Krumholz Laboratory for Advanced Microscopy at the Technion, part of the Technion Project on Complex Fluids.

PY - 2007/10

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N2 - Cholesterol gallstones usually develop in the gallbladder and rarely form in bile ducts even in patients with highly lithogenic bile. Bile concentration and proteins (e.g. mucin) may affect crystallization, but the exact nature of this effect, especially in relation to crystallization pathways and microstructural evolution remains unclear. We examined lipid microstructures in paired hepatic and gallbladder biles to reveal ones that are essential for crystallization. Combining digital light microscopy with cryogenic-temperature transmission electron microscopy we are able to directly visualize and compare the time evolution of lipid microstructures in paired hepatic, gallbladder and diluted gallbladder biles of gallstone patients and controls, without drying or separating. Gallbladder bile exhibited several multilamellar vesicles and spheroidal micelles preceding and throughout crystallization. Vesicle morphology changed before crystallization was observed. In contrast, hepatic bile revealed almost no crystallization and while a variety of unilamellar vesicles and spheroidal micelles existed throughout the examination, multilamellar vesicles were rare. Diluted gallbladder bile was different from native gallbladder bile, as well as the paired hepatic bile, yielding occasional crystallization. Our findings suggest that maturing multilamellar vesicles precede (and at least partially initiate) crystallization in gallbladder bile. Although microstructural development seems to be concentration dependent, dilution of gallbladder bile to hepatic bile concentrations neither makes it identical to hepatic bile, nor prevents crystallization.

AB - Cholesterol gallstones usually develop in the gallbladder and rarely form in bile ducts even in patients with highly lithogenic bile. Bile concentration and proteins (e.g. mucin) may affect crystallization, but the exact nature of this effect, especially in relation to crystallization pathways and microstructural evolution remains unclear. We examined lipid microstructures in paired hepatic and gallbladder biles to reveal ones that are essential for crystallization. Combining digital light microscopy with cryogenic-temperature transmission electron microscopy we are able to directly visualize and compare the time evolution of lipid microstructures in paired hepatic, gallbladder and diluted gallbladder biles of gallstone patients and controls, without drying or separating. Gallbladder bile exhibited several multilamellar vesicles and spheroidal micelles preceding and throughout crystallization. Vesicle morphology changed before crystallization was observed. In contrast, hepatic bile revealed almost no crystallization and while a variety of unilamellar vesicles and spheroidal micelles existed throughout the examination, multilamellar vesicles were rare. Diluted gallbladder bile was different from native gallbladder bile, as well as the paired hepatic bile, yielding occasional crystallization. Our findings suggest that maturing multilamellar vesicles precede (and at least partially initiate) crystallization in gallbladder bile. Although microstructural development seems to be concentration dependent, dilution of gallbladder bile to hepatic bile concentrations neither makes it identical to hepatic bile, nor prevents crystallization.

KW - Cholesterol gallstones

KW - Cryogenic-temperature transmission electron microscopy

KW - Digital light microscopy

KW - Lipid microstructures

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DO - 10.1016/j.bbalip.2007.07.006

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AN - SCOPUS:34848829765

VL - 1771

SP - 1289

EP - 1298

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 10

ER -

A comparative study of microstructural development in paired human hepatic and gallbladder biles

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Keywords

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