Unexpectedly high diversity of anammox bacteria detected in deep-sea surface sediments of the South China Sea

TY - JOUR

T1 - Unexpectedly high diversity of anammox bacteria detected in deep-sea surface sediments of the South China Sea

AU - Wu, Jiapeng

AU - Hong, Yiguo

AU - Chang, Xiangyang

AU - Jiao, Lijing

AU - Li, Yiben

AU - Liu, Xiaohan

AU - Xie, Haitao

AU - Gu, Ji Dong

N1 - Publisher Copyright: © FEMS 2019. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Candidatus Scalindua is an exclusive genus of anammox bacteria known to exhibit low diversity found in deep-sea ecosystems. In this study, the community composition of anammox bacteria in surface sediments of the South China Sea was analyzed using high-throughput sequencing techniques. Results indicated that the dominant operational taxonomic units were related to three different genera of anammox bacteria, identified as Ca. Scalindua (87.29%), Ca. Brocadia (10.27%) and Ca. Kuenenia (2.44%), in order of decreasing abundance. Quantitative PCR analysis of anammox-specific 16S rRNA and hzsB genes confirmed that the abundance of anammox bacteria in deep-sea surface sediments ranged from 4.34 × 105 to 3.91 × 107 and 1.62 × 105 to 1.63 × 108 copies per gram, respectively. The ACE, Chao1 and Shannon estimators for anammox bacteria were significantly higher than those reported in previous studies. Pearson correlation and redundancy analyses indicated that depth and temperature were the key factors affecting the distribution, abundance and diversity of anammox bacteria in deep-sea sediments. We herein report the wide distribution of Ca. Kuenenia and Ca. Brocadia in deep-sea sediments and provide comprehensive information on the distribution and ecological significance of anammox bacteria in deep-sea environments.

AB - Candidatus Scalindua is an exclusive genus of anammox bacteria known to exhibit low diversity found in deep-sea ecosystems. In this study, the community composition of anammox bacteria in surface sediments of the South China Sea was analyzed using high-throughput sequencing techniques. Results indicated that the dominant operational taxonomic units were related to three different genera of anammox bacteria, identified as Ca. Scalindua (87.29%), Ca. Brocadia (10.27%) and Ca. Kuenenia (2.44%), in order of decreasing abundance. Quantitative PCR analysis of anammox-specific 16S rRNA and hzsB genes confirmed that the abundance of anammox bacteria in deep-sea surface sediments ranged from 4.34 × 105 to 3.91 × 107 and 1.62 × 105 to 1.63 × 108 copies per gram, respectively. The ACE, Chao1 and Shannon estimators for anammox bacteria were significantly higher than those reported in previous studies. Pearson correlation and redundancy analyses indicated that depth and temperature were the key factors affecting the distribution, abundance and diversity of anammox bacteria in deep-sea sediments. We herein report the wide distribution of Ca. Kuenenia and Ca. Brocadia in deep-sea sediments and provide comprehensive information on the distribution and ecological significance of anammox bacteria in deep-sea environments.

KW - Anammox

KW - Community structure

KW - Deep sea

KW - Diversity

KW - South China Sea

UR - http://www.scopus.com/inward/record.url?scp=85062010509&partnerID=8YFLogxK

U2 - 10.1093/femsec/fiz013

DO - 10.1093/femsec/fiz013

M3 - 文章

C2 - 30668684

AN - SCOPUS:85062010509

SN - 0168-6496

VL - 95

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

IS - 3

M1 - fiy013

ER -