Stable nanovesicles formed by intrinsically planar bilayers

Mariana Köber; Sílvia Illa-Tuset; Lidia Ferrer-Tasies; Evelyn Moreno-Calvo; Witold I. Tatkiewicz; Natascia Grimaldi; David Piña; Alejandro Pérez Pérez; Vega Lloveras; José Vidal-Gancedo; Donatella Bulone; Imma Ratera; Jan Skov Pedersen; Dganit Danino; Jaume Veciana; Jordi Faraudo; Nora Ventosa

doi:10.1016/j.jcis.2022.10.104

Stable nanovesicles formed by intrinsically planar bilayers

Mariana Köber, Sílvia Illa-Tuset, Lidia Ferrer-Tasies, Evelyn Moreno-Calvo, Witold I. Tatkiewicz, Natascia Grimaldi, David Piña, Alejandro Pérez Pérez, Vega Lloveras, José Vidal-Gancedo, Donatella Bulone, Imma Ratera, Jan Skov Pedersen, Dganit Danino, Jaume Veciana, Jordi Faraudo^*, Nora Ventosa

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Hypothesis: Quatsome nanovesicles, formed through the self-assembly of cholesterol (CHOL) and cetyltrimethylammonium bromide (CTAB) in water, have shown long-term stability in terms of size and morphology, while at the same time exhibiting high CHOL-CTAB intermolecular binding energies. We hypothesize that CHOL/CTAB quatsomes are indeed thermodynamically stable nanovesicles, and investigate the mechanism underlying their formation. Experiments: A systematic study was performed to determine whether CHOL/CTAB quatsomes satisfy the experimental requisites of thermodynamically stable vesicles. Coarse-grain molecular dynamics simulations were used to investigate the molecular organization in the vesicle membrane, and the characteristics of the simulated vesicle were corroborated with experimental data obtained by cryo–electron microscopy, small- and wide-angle X-ray scattering, and multi-angle static light scattering. Findings: CHOL/CTAB quatsomes fulfill the requisites of thermodynamically stable nanovesicles, but they do not exhibit the classical membrane curvature induced by a composition asymmetry between the bilayer leaflets, like catanionic nanovesicles. Instead, CHOL/CTAB quatsomes are formed through the association of intrinsically planar bilayers in a faceted vesicle with defects, indicating that distortions in the organization and orientation of molecules can play a major role in the formation of thermodynamically stable nanovesicles.

Original language	English
Pages (from-to)	202-211
Number of pages	10
Journal	Journal of Colloid and Interface Science
Volume	631
DOIs	https://doi.org/10.1016/j.jcis.2022.10.104
State	Published - Feb 2023
Externally published	Yes

Keywords

Composition asymmetry
Molecular self-assembly
Nanovesicles
Quatsomes
Vesicle stability

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10.1016/j.jcis.2022.10.104

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Köber, M., Illa-Tuset, S., Ferrer-Tasies, L., Moreno-Calvo, E., Tatkiewicz, W. I., Grimaldi, N., Piña, D., Pérez, A. P., Lloveras, V., Vidal-Gancedo, J., Bulone, D., Ratera, I., Pedersen, J. S., Danino, D., Veciana, J., Faraudo, J., & Ventosa, N. (2023). Stable nanovesicles formed by intrinsically planar bilayers. Journal of Colloid and Interface Science, 631, 202-211. https://doi.org/10.1016/j.jcis.2022.10.104

@article{55ae2f66e79b45ccb06c2ac5041e56b5,

title = "Stable nanovesicles formed by intrinsically planar bilayers",

abstract = "Hypothesis: Quatsome nanovesicles, formed through the self-assembly of cholesterol (CHOL) and cetyltrimethylammonium bromide (CTAB) in water, have shown long-term stability in terms of size and morphology, while at the same time exhibiting high CHOL-CTAB intermolecular binding energies. We hypothesize that CHOL/CTAB quatsomes are indeed thermodynamically stable nanovesicles, and investigate the mechanism underlying their formation. Experiments: A systematic study was performed to determine whether CHOL/CTAB quatsomes satisfy the experimental requisites of thermodynamically stable vesicles. Coarse-grain molecular dynamics simulations were used to investigate the molecular organization in the vesicle membrane, and the characteristics of the simulated vesicle were corroborated with experimental data obtained by cryo–electron microscopy, small- and wide-angle X-ray scattering, and multi-angle static light scattering. Findings: CHOL/CTAB quatsomes fulfill the requisites of thermodynamically stable nanovesicles, but they do not exhibit the classical membrane curvature induced by a composition asymmetry between the bilayer leaflets, like catanionic nanovesicles. Instead, CHOL/CTAB quatsomes are formed through the association of intrinsically planar bilayers in a faceted vesicle with defects, indicating that distortions in the organization and orientation of molecules can play a major role in the formation of thermodynamically stable nanovesicles.",

keywords = "Composition asymmetry, Molecular self-assembly, Nanovesicles, Quatsomes, Vesicle stability",

author = "Mariana K{\"o}ber and S{\'i}lvia Illa-Tuset and Lidia Ferrer-Tasies and Evelyn Moreno-Calvo and Tatkiewicz, {Witold I.} and Natascia Grimaldi and David Pi{\~n}a and P{\'e}rez, {Alejandro P{\'e}rez} and Vega Lloveras and Jos{\'e} Vidal-Gancedo and Donatella Bulone and Imma Ratera and Pedersen, {Jan Skov} and Dganit Danino and Jaume Veciana and Jordi Faraudo and Nora Ventosa",

note = "Publisher Copyright: {\textcopyright} 2022",

year = "2023",

month = feb,

doi = "10.1016/j.jcis.2022.10.104",

language = "英语",

volume = "631",

pages = "202--211",

journal = "Journal of Colloid and Interface Science",

issn = "0021-9797",

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Köber, M, Illa-Tuset, S, Ferrer-Tasies, L, Moreno-Calvo, E, Tatkiewicz, WI, Grimaldi, N, Piña, D, Pérez, AP, Lloveras, V, Vidal-Gancedo, J, Bulone, D, Ratera, I, Pedersen, JS, Danino, D, Veciana, J, Faraudo, J & Ventosa, N 2023, 'Stable nanovesicles formed by intrinsically planar bilayers', Journal of Colloid and Interface Science, vol. 631, pp. 202-211. https://doi.org/10.1016/j.jcis.2022.10.104

TY - JOUR

T1 - Stable nanovesicles formed by intrinsically planar bilayers

AU - Köber, Mariana

AU - Illa-Tuset, Sílvia

AU - Ferrer-Tasies, Lidia

AU - Moreno-Calvo, Evelyn

AU - Tatkiewicz, Witold I.

AU - Grimaldi, Natascia

AU - Piña, David

AU - Pérez, Alejandro Pérez

AU - Lloveras, Vega

AU - Vidal-Gancedo, José

AU - Bulone, Donatella

AU - Ratera, Imma

AU - Pedersen, Jan Skov

AU - Danino, Dganit

AU - Veciana, Jaume

AU - Faraudo, Jordi

AU - Ventosa, Nora

PY - 2023/2

Y1 - 2023/2

N2 - Hypothesis: Quatsome nanovesicles, formed through the self-assembly of cholesterol (CHOL) and cetyltrimethylammonium bromide (CTAB) in water, have shown long-term stability in terms of size and morphology, while at the same time exhibiting high CHOL-CTAB intermolecular binding energies. We hypothesize that CHOL/CTAB quatsomes are indeed thermodynamically stable nanovesicles, and investigate the mechanism underlying their formation. Experiments: A systematic study was performed to determine whether CHOL/CTAB quatsomes satisfy the experimental requisites of thermodynamically stable vesicles. Coarse-grain molecular dynamics simulations were used to investigate the molecular organization in the vesicle membrane, and the characteristics of the simulated vesicle were corroborated with experimental data obtained by cryo–electron microscopy, small- and wide-angle X-ray scattering, and multi-angle static light scattering. Findings: CHOL/CTAB quatsomes fulfill the requisites of thermodynamically stable nanovesicles, but they do not exhibit the classical membrane curvature induced by a composition asymmetry between the bilayer leaflets, like catanionic nanovesicles. Instead, CHOL/CTAB quatsomes are formed through the association of intrinsically planar bilayers in a faceted vesicle with defects, indicating that distortions in the organization and orientation of molecules can play a major role in the formation of thermodynamically stable nanovesicles.

AB - Hypothesis: Quatsome nanovesicles, formed through the self-assembly of cholesterol (CHOL) and cetyltrimethylammonium bromide (CTAB) in water, have shown long-term stability in terms of size and morphology, while at the same time exhibiting high CHOL-CTAB intermolecular binding energies. We hypothesize that CHOL/CTAB quatsomes are indeed thermodynamically stable nanovesicles, and investigate the mechanism underlying their formation. Experiments: A systematic study was performed to determine whether CHOL/CTAB quatsomes satisfy the experimental requisites of thermodynamically stable vesicles. Coarse-grain molecular dynamics simulations were used to investigate the molecular organization in the vesicle membrane, and the characteristics of the simulated vesicle were corroborated with experimental data obtained by cryo–electron microscopy, small- and wide-angle X-ray scattering, and multi-angle static light scattering. Findings: CHOL/CTAB quatsomes fulfill the requisites of thermodynamically stable nanovesicles, but they do not exhibit the classical membrane curvature induced by a composition asymmetry between the bilayer leaflets, like catanionic nanovesicles. Instead, CHOL/CTAB quatsomes are formed through the association of intrinsically planar bilayers in a faceted vesicle with defects, indicating that distortions in the organization and orientation of molecules can play a major role in the formation of thermodynamically stable nanovesicles.

KW - Composition asymmetry

KW - Molecular self-assembly

KW - Nanovesicles

KW - Quatsomes

KW - Vesicle stability

UR - http://www.scopus.com/inward/record.url?scp=85141516433&partnerID=8YFLogxK

U2 - 10.1016/j.jcis.2022.10.104

DO - 10.1016/j.jcis.2022.10.104

M3 - 文章

C2 - 36375300

AN - SCOPUS:85141516433

SN - 0021-9797

VL - 631

SP - 202

EP - 211

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

ER -

Stable nanovesicles formed by intrinsically planar bilayers

Abstract

Keywords

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