Spatial distribution and abundance of ammonia-oxidizing microorganisms in deep-sea sediments of the Pacific Ocean

TY - JOUR

T1 - Spatial distribution and abundance of ammonia-oxidizing microorganisms in deep-sea sediments of the Pacific Ocean

AU - Luo, Zhu Hua

AU - Xu, Wei

AU - Li, Meng

AU - Gu, Ji Dong

AU - Zhong, Tian Hua

N1 - Publisher Copyright: © Springer International Publishing Switzerland 2015.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Nitrification, the aerobic oxidation of ammonia to nitrate via nitrite, is performed by nitrifying microbes including ammonia-oxidizing bacteria (AOB) and archaea (AOA). In the current study, the phylogenetic diversity and abundance of AOB and AOA in deep-sea sediments of the Pacific Ocean were investigated using ammonia monooxygenase subunit A (amoA) coding genes as molecular markers. The study uncovered 3 AOB unique operational taxonomic units (OTUs, defined at sequence groups that differ by ≤5 %), which indicates lower diversity than AOA (13 OTUs obtained). All AOB amoA gene sequences were phylogenetically related to amoA sequences similar to those found in marine Nitrosospira species, and all AOA amoA gene sequences were affiliated with the marine sediment clade. Quantitative PCR revealed similar archaeal amoA gene abundances [1.68 × 105–1.89 × 106 copies/g sediment (wet weight)] among different sites. Bacterial amoA gene abundances ranged from 5.28 × 103 to 2.29 × 106 copies/g sediment (wet weight). The AOA/AOB amoA gene abundance ratios ranged from 0.012 to 162 and were negatively correlated with total C and C/N ratio. These results suggest that organic loading may be a key factor regulating the relative abundance of AOA and AOB in deep-sea environments of the Pacific Ocean.

AB - Nitrification, the aerobic oxidation of ammonia to nitrate via nitrite, is performed by nitrifying microbes including ammonia-oxidizing bacteria (AOB) and archaea (AOA). In the current study, the phylogenetic diversity and abundance of AOB and AOA in deep-sea sediments of the Pacific Ocean were investigated using ammonia monooxygenase subunit A (amoA) coding genes as molecular markers. The study uncovered 3 AOB unique operational taxonomic units (OTUs, defined at sequence groups that differ by ≤5 %), which indicates lower diversity than AOA (13 OTUs obtained). All AOB amoA gene sequences were phylogenetically related to amoA sequences similar to those found in marine Nitrosospira species, and all AOA amoA gene sequences were affiliated with the marine sediment clade. Quantitative PCR revealed similar archaeal amoA gene abundances [1.68 × 105–1.89 × 106 copies/g sediment (wet weight)] among different sites. Bacterial amoA gene abundances ranged from 5.28 × 103 to 2.29 × 106 copies/g sediment (wet weight). The AOA/AOB amoA gene abundance ratios ranged from 0.012 to 162 and were negatively correlated with total C and C/N ratio. These results suggest that organic loading may be a key factor regulating the relative abundance of AOA and AOB in deep-sea environments of the Pacific Ocean.

KW - Abundance

KW - Ammonia monooxygenase α-subunit (amoA) gene

KW - Ammonia-oxidizing archaea

KW - Ammonia-oxidizing bacteria

KW - Deep-sea sediments

KW - Diversity

UR - http://www.scopus.com/inward/record.url?scp=84943357560&partnerID=8YFLogxK

U2 - 10.1007/s10482-015-0485-4

DO - 10.1007/s10482-015-0485-4

M3 - 文章

C2 - 26014493

AN - SCOPUS:84943357560

SN - 0003-6072

VL - 108

SP - 329

EP - 342

JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

IS - 2

M1 - A005

ER -