Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution

TY - JOUR

T1 - Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution

AU - Liu, Xiao Bo

AU - Gu, Qiu Ya

AU - Yu, Xiao Bin

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (Grant No. 21176105 ), Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (Grant No. KLIB-KF201105 ), the Fundamental Research Funds for the Central Universities (Grant No. JUSRP111A24 ), the 111 Project (No. 111-2-06 ) and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions .

PY - 2013/2

Y1 - 2013/2

N2 - To improve butanol tolerance and production in Clostridium acetobutylicum, a novel approach was developed in this study, which was called artificial simulation of bio-evolution (ASBE) based on the evolutionary dynamics and natural selection. Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2. g/L to 15.3. g/L using corn meal as substrate. Fermentation was also carried out to investigate the relationship between butanol tolerance and ABE production, suggesting that enhancing butanol tolerance could increase butanol production but unlikely improve total ABE production. These results also indicated that the ASBE would be an available and feasible method used in biotechnology for enhancement of butanol tolerance and production.

AB - To improve butanol tolerance and production in Clostridium acetobutylicum, a novel approach was developed in this study, which was called artificial simulation of bio-evolution (ASBE) based on the evolutionary dynamics and natural selection. Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2. g/L to 15.3. g/L using corn meal as substrate. Fermentation was also carried out to investigate the relationship between butanol tolerance and ABE production, suggesting that enhancing butanol tolerance could increase butanol production but unlikely improve total ABE production. These results also indicated that the ASBE would be an available and feasible method used in biotechnology for enhancement of butanol tolerance and production.

KW - ABE production

KW - Biofuel

KW - Butanol tolerance

KW - Clostridium acetobutylicum

KW - Domestication

UR - http://www.scopus.com/inward/record.url?scp=84872404411&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2012.12.121

DO - 10.1016/j.biortech.2012.12.121

M3 - 文章

C2 - 23334021

AN - SCOPUS:84872404411

SN - 0960-8524

VL - 130

SP - 638

EP - 643

JO - Bioresource Technology

JF - Bioresource Technology

ER -