Protection of catheter surfaces from adhesion of Pseudomonas aeruginosa by a combination of silver ions and lectins

TY - JOUR

T1 - Protection of catheter surfaces from adhesion of Pseudomonas aeruginosa by a combination of silver ions and lectins

AU - Gu, Ji Dong

AU - Belay, Brook

AU - Mitchell, Ralph

N1 - Funding Information: Preparation of this manuscript was partially supported by a CRCG grant from The University of Hong Kong (to J-DG). We would like to thank R. Penny for reading the manuscript.

PY - 2001

Y1 - 2001

N2 - A catheter surface was modified by coating a cellulose acetate polymer. Adhesion of Pseudomonas aeruginosa ATCC 27853 to the surface was investigated by exposing bacterial cultures to three treatments: polymer impregnated with silver ions (Ag+), polymer surfaces coated with lectins and a combination of Ag+ and a lectin coating. The effective concentration of Ag+ providing protection against bacterial biofilm development was 100 μg/ml and higher. Lectins alone at 10% also showed inhibition of bacterial attachment. However, the best result was achieved against bacterial adhesion and growth on surfaces using a combination of 100 μg Ag+/ml and a lectin coating as a surface treatment. This surface treatment was also effective against both fresh culture and a two-week-old culture containing P. aeruginosa producing exopolymers. Our results suggest that Ag+ impregnation combined with a lectin coating warrants further investigation as a potential means of protecting catheters.

AB - A catheter surface was modified by coating a cellulose acetate polymer. Adhesion of Pseudomonas aeruginosa ATCC 27853 to the surface was investigated by exposing bacterial cultures to three treatments: polymer impregnated with silver ions (Ag+), polymer surfaces coated with lectins and a combination of Ag+ and a lectin coating. The effective concentration of Ag+ providing protection against bacterial biofilm development was 100 μg/ml and higher. Lectins alone at 10% also showed inhibition of bacterial attachment. However, the best result was achieved against bacterial adhesion and growth on surfaces using a combination of 100 μg Ag+/ml and a lectin coating as a surface treatment. This surface treatment was also effective against both fresh culture and a two-week-old culture containing P. aeruginosa producing exopolymers. Our results suggest that Ag+ impregnation combined with a lectin coating warrants further investigation as a potential means of protecting catheters.

KW - Adhesion

KW - Biofilms

KW - Catheter

KW - Lectin

KW - Pseudomonas aeruginosa

KW - Silver ions

UR - http://www.scopus.com/inward/record.url?scp=0035745387&partnerID=8YFLogxK

U2 - 10.1023/A:1016687630330

DO - 10.1023/A:1016687630330

M3 - 文章

AN - SCOPUS:0035745387

SN - 0959-3993

VL - 17

SP - 173

EP - 179

JO - World Journal of Microbiology and Biotechnology

JF - World Journal of Microbiology and Biotechnology

IS - 2

ER -