TY - JOUR
T1 - Phylogenetic diversity and axial distribution of microbes in the intestinal tract of the polychaete Neanthes glandicincta
AU - Li, Meng
AU - Yang, Hong
AU - Gu, Ji Dong
N1 - Funding Information:
We would like to express our sincere thanks to Dr. Zhenye Zhao and Ms. Jessie Lai at The University of Hong Kong for providing samples and technical assistance in the field. We also like to thank Adam Mumford at The State University of New Jersey for his kind review and comments on this manuscript. This work was supported in part by a research grant CA04/05.SC01 from Research Grant Council of Hong Kong Government (J-DG) and project no. 30570034 from NSFC (HY).
PY - 2009/11
Y1 - 2009/11
N2 - The phylogenetic diversity and axial distribution of microorganisms in three sections of the gastrointestinal tracts of the polychaete Neanthes glandicincta was evaluated using both most probable number method and cloning analyses of 16S rRNA genes in this study. Quantification of the density of microorganisms in the gut showed that aerobic microorganisms decreased from anterior to posterior, while anaerobic ones showed a reverse trend. The total numbers of microorganisms decreased significantly (p<0.05, analysis of variance) but more rapidly from the anterior to the middle segment. Phylogenetic analysis showed that the dominating phylogenetic groups included Methanomicrobiales I: Methanosaetaceae (up to 66% of archaeal clones), δ-Proteobacteria (up to 42% of bacterial clones), and γ-Proteobacteria (up to 30% of bacterial clones) widely distributed throughout the entire gut. Other microbiota distributed in different gut sections were Methanomicrobiales II: Methanospirillaceae, Methanomicrobiales III, Thermoplasmatales, Crenarchaea, Methanobacteriaceae, and Methanosarcinales for archaea; and α-Proteobacteria, β-Proteobacteria, Fusobacteria, Clostridia, Chloroflexi, and Planctomycetes for bacteria. The results reveal a difference in microbial community structure along the gut of N. glandicincta. The various phylogenetic diversity and axial distribution of microbes along the gut might indicate an environmental gradient from anterior to posterior sections affecting the structure of the microbial community.
AB - The phylogenetic diversity and axial distribution of microorganisms in three sections of the gastrointestinal tracts of the polychaete Neanthes glandicincta was evaluated using both most probable number method and cloning analyses of 16S rRNA genes in this study. Quantification of the density of microorganisms in the gut showed that aerobic microorganisms decreased from anterior to posterior, while anaerobic ones showed a reverse trend. The total numbers of microorganisms decreased significantly (p<0.05, analysis of variance) but more rapidly from the anterior to the middle segment. Phylogenetic analysis showed that the dominating phylogenetic groups included Methanomicrobiales I: Methanosaetaceae (up to 66% of archaeal clones), δ-Proteobacteria (up to 42% of bacterial clones), and γ-Proteobacteria (up to 30% of bacterial clones) widely distributed throughout the entire gut. Other microbiota distributed in different gut sections were Methanomicrobiales II: Methanospirillaceae, Methanomicrobiales III, Thermoplasmatales, Crenarchaea, Methanobacteriaceae, and Methanosarcinales for archaea; and α-Proteobacteria, β-Proteobacteria, Fusobacteria, Clostridia, Chloroflexi, and Planctomycetes for bacteria. The results reveal a difference in microbial community structure along the gut of N. glandicincta. The various phylogenetic diversity and axial distribution of microbes along the gut might indicate an environmental gradient from anterior to posterior sections affecting the structure of the microbial community.
UR - http://www.scopus.com/inward/record.url?scp=73349088760&partnerID=8YFLogxK
U2 - 10.1007/s00248-009-9550-8
DO - 10.1007/s00248-009-9550-8
M3 - 文章
C2 - 19572164
AN - SCOPUS:73349088760
SN - 0095-3628
VL - 58
SP - 892
EP - 902
JO - Microbial Ecology
JF - Microbial Ecology
IS - 4
ER -