TY - JOUR
T1 - Optimization of liquid media and biosafety assessment for algae-lysing bacterium NP23
AU - Liao, Chunli
AU - Liu, Xiaobo
AU - Shan, Linna
N1 - Publisher Copyright:
© 2014, NRC Research Press. All rights reserved.
PY - 2014/7/1
Y1 - 2014/7/1
N2 - To control algal bloom caused by nutrient pollution, a wild-type algae-lysing bacterium was isolated from the Baiguishan reservoir in Henan province of China and identified as Enterobacter sp. strain NP23. Algal culture medium was optimized by applying a Placket–Burman design to obtain a high cell concentration of NP23. Three minerals (i.e., 0.6% KNO3, 0.001% MnSO4·H2O, and 0.3% K2HPO4) were found to be independent factors critical for obtaining the highest cell concentration of 1013 CFU/mL, which was 104 times that of the control. In the algae-lysing experiment, the strain exhibited a high lysis rate for the 4 algae test species, namely, Chlorella vulgari, Scenedesmus, Microcystis wesenbergii, and Chlorella pyrenoidosa. Acute toxicity and mutagenicity tests showed that the bacterium NP23 had no toxic and mutagenic effects on fish, even in large doses such as 107 or 109 CFU/mL. Thus, Enterobacter sp. strain NP23 has strong potential application in the microbial algae-lysing project.
AB - To control algal bloom caused by nutrient pollution, a wild-type algae-lysing bacterium was isolated from the Baiguishan reservoir in Henan province of China and identified as Enterobacter sp. strain NP23. Algal culture medium was optimized by applying a Placket–Burman design to obtain a high cell concentration of NP23. Three minerals (i.e., 0.6% KNO3, 0.001% MnSO4·H2O, and 0.3% K2HPO4) were found to be independent factors critical for obtaining the highest cell concentration of 1013 CFU/mL, which was 104 times that of the control. In the algae-lysing experiment, the strain exhibited a high lysis rate for the 4 algae test species, namely, Chlorella vulgari, Scenedesmus, Microcystis wesenbergii, and Chlorella pyrenoidosa. Acute toxicity and mutagenicity tests showed that the bacterium NP23 had no toxic and mutagenic effects on fish, even in large doses such as 107 or 109 CFU/mL. Thus, Enterobacter sp. strain NP23 has strong potential application in the microbial algae-lysing project.
KW - Acute toxicity and mutagenicity tests
KW - Algae-lysing bacterium
KW - Algal bloom
KW - Plackett–Burman design
UR - http://www.scopus.com/inward/record.url?scp=84906966557&partnerID=8YFLogxK
U2 - 10.1139/cjm-2014-0322
DO - 10.1139/cjm-2014-0322
M3 - 文章
C2 - 25188453
AN - SCOPUS:84906966557
SN - 0008-4166
VL - 60
SP - 593
EP - 597
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
IS - 9
ER -