Nitrogen removal properties in a continuous marine anammox bacteria reactor under rapid and extensive salinity changes

TY - JOUR

T1 - Nitrogen removal properties in a continuous marine anammox bacteria reactor under rapid and extensive salinity changes

AU - Wei, Qiaoyan

AU - Kawagoshi, Yasunori

AU - Huang, Xiaowu

AU - Hong, Nian

AU - Van Duc, Luong

AU - Yamashita, Yuki

AU - Hama, Takehide

N1 - Publisher Copyright: © 2016 Elsevier Ltd.

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Salinity tolerance is one of the most important factors for the application of bioreactors to high-salinity wastewater. Although marine anammox bacteria (MAB) might be expected to tolerate higher salinities than freshwater anammox bacteria, there is little information on the effects of salinity on MAB activity. This study aimed to reveal the nitrogen removal properties in a continuous MAB reactor under conditions of rapid and extensive salinity changes. The reactor demonstrated stable nitrogen removal performance with a removal efficiency of over 85% under salinity conditions ranging from 0 to 50 g/L NaCl. The reactor performance was also well maintained, even though the salinity was rapidly changed from 30 to 50 g/L and from 30 to 0 g/L. Other evidence suggested that the seawater medium used contained components essential for effective MAB performance. Bacterial community analysis using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) showed that planctomycete UKU-1, the dominant MAB species in the inoculum, was the main contributor to anammox activity under all conditions. The PCR-DGGE using a universal bacterial primer set showed different DNA band patterns between the reactor biomass sample collected under conditions of 75 g/L NaCl and all other conditions (0, 30, 50 and freshwater-medium). All DNA sequences determined were very similar to those of bacterial species from marine environments, anaerobic environments, or wastewater-treatment facilities.

AB - Salinity tolerance is one of the most important factors for the application of bioreactors to high-salinity wastewater. Although marine anammox bacteria (MAB) might be expected to tolerate higher salinities than freshwater anammox bacteria, there is little information on the effects of salinity on MAB activity. This study aimed to reveal the nitrogen removal properties in a continuous MAB reactor under conditions of rapid and extensive salinity changes. The reactor demonstrated stable nitrogen removal performance with a removal efficiency of over 85% under salinity conditions ranging from 0 to 50 g/L NaCl. The reactor performance was also well maintained, even though the salinity was rapidly changed from 30 to 50 g/L and from 30 to 0 g/L. Other evidence suggested that the seawater medium used contained components essential for effective MAB performance. Bacterial community analysis using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) showed that planctomycete UKU-1, the dominant MAB species in the inoculum, was the main contributor to anammox activity under all conditions. The PCR-DGGE using a universal bacterial primer set showed different DNA band patterns between the reactor biomass sample collected under conditions of 75 g/L NaCl and all other conditions (0, 30, 50 and freshwater-medium). All DNA sequences determined were very similar to those of bacterial species from marine environments, anaerobic environments, or wastewater-treatment facilities.

KW - Anammox

KW - High-salinity tolerance

KW - Marine anammox bacteria

KW - Nitrogen removal performance

KW - Reactor

UR - http://www.scopus.com/inward/record.url?scp=84960423071&partnerID=8YFLogxK

U2 - 10.1016/j.chemosphere.2016.01.041

DO - 10.1016/j.chemosphere.2016.01.041

M3 - 文章

C2 - 26845464

AN - SCOPUS:84960423071

SN - 0045-6535

VL - 148

SP - 444

EP - 451

JO - Chemosphere

JF - Chemosphere

ER -