TY - JOUR
T1 - Molecular marker assisted selection for improvement of the eating, cooking and sensory quality of rice (Oryza sativa L.)
AU - Jin, Liang
AU - Lu, Yan
AU - Shao, Yafang
AU - Zhang, Gan
AU - Xiao, Peng
AU - Shen, Shengquan
AU - Corke, Harold
AU - Bao, Jinsong
N1 - Funding Information:
We thank the anonymous reviewers for their constructive comments. Financial support for this research was provided in part by National High Technology Development Project of China (2006AA10Z193), National Natural Science Foundation of China (30300227, 30771327), the Science and Technology Department of Zhejiang Province (2007C32014), International Atomic Energy Agency (12847/R0), and a Hong Kong Research Grants Council GRF award 2006 (7507/06M).
PY - 2010/1
Y1 - 2010/1
N2 - II-32B is a key maintainer line used for hybrid rice breeding in China. However, it is of poor quality for most Chinese consumers because of its high apparent amylose content (AAC), high gelatinization temperature (GT) and non-fragrance. It is well known that the AAC, GT and fragrance traits are largely controlled by the Wx, starch synthase IIa (SSIIa), and fragrance (fgr) genes, respectively. With the aid of functional markers, we improved the quality of II-32B by introgressing the Wx, SSIIa, and fgr genes from Yixiang B, a fragrant maintainer line that has low AAC and low GT. The microsatellite allele (CT)17 of Wx, the contiguous single nucleotide polymorphism TT allele of SSIIa and the 8-bp deletion allele of fgr were transferred to II-32B by two backcrosses and three selfings. Molecular marker assisted selection was applied in the series to select for individuals carrying Wx-(CT)17, SSIIa-TT, and fgr-deletion alleles. According to the marker genotypes, seventeen homozygous lines for Wx-(CT)17, SSIIa-TT, and fgr gene markers were finally selected. The improved II-32B lines were fragrant with reduced AAC and GT.
AB - II-32B is a key maintainer line used for hybrid rice breeding in China. However, it is of poor quality for most Chinese consumers because of its high apparent amylose content (AAC), high gelatinization temperature (GT) and non-fragrance. It is well known that the AAC, GT and fragrance traits are largely controlled by the Wx, starch synthase IIa (SSIIa), and fragrance (fgr) genes, respectively. With the aid of functional markers, we improved the quality of II-32B by introgressing the Wx, SSIIa, and fgr genes from Yixiang B, a fragrant maintainer line that has low AAC and low GT. The microsatellite allele (CT)17 of Wx, the contiguous single nucleotide polymorphism TT allele of SSIIa and the 8-bp deletion allele of fgr were transferred to II-32B by two backcrosses and three selfings. Molecular marker assisted selection was applied in the series to select for individuals carrying Wx-(CT)17, SSIIa-TT, and fgr-deletion alleles. According to the marker genotypes, seventeen homozygous lines for Wx-(CT)17, SSIIa-TT, and fgr gene markers were finally selected. The improved II-32B lines were fragrant with reduced AAC and GT.
KW - Marker assisted selection
KW - Quality
KW - Rice
KW - Wx gene
UR - http://www.scopus.com/inward/record.url?scp=73549107010&partnerID=8YFLogxK
U2 - 10.1016/j.jcs.2009.11.007
DO - 10.1016/j.jcs.2009.11.007
M3 - 文章
AN - SCOPUS:73549107010
SN - 0733-5210
VL - 51
SP - 159
EP - 164
JO - Journal of Cereal Science
JF - Journal of Cereal Science
IS - 1
ER -