Molecular-level characterization of stratified extracellular polymeric substances of anammox sludge and its adsorption preference to refractory dissolved organic matter

TY - JOUR

T1 - Molecular-level characterization of stratified extracellular polymeric substances of anammox sludge and its adsorption preference to refractory dissolved organic matter

AU - Zhang, Li

AU - Peng, Yongzhen

AU - Soda, Satoshi

AU - Huang, Xiaowu

AU - Wang, Yifei

AU - Zhang, Yanan

N1 - Publisher Copyright: © 2020 Elsevier Ltd

PY - 2020/12/15

Y1 - 2020/12/15

N2 - Anaerobic ammonium oxidation (anammox) is an energy saving and environmentally friendly technique for wastewater treatment. Sludge adsorption is an important process after organics enter the anammox reactor. The extracellular polymeric substances (EPS) of anammox sludge play a key role on the activity of anammox. This study utilized Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) to investigate molecular-level compositional characteristics of the stratified EPS of the anammox sludge, and to explore the adsorption preference of each EPS layer to refractory dissolved organic matter (DOM) during anammox treatment. Results showed that the adsorbed component by the tightly bound EPS layer was dominantly composed of lipids and proteins, with nearly 80% of formulas being CHO and CHON. The outer layers (slime, loosely bound-EPS) preferentially adsorbed the compounds with comparatively higher aromatic and unsaturated degrees, with S-containing formulas and lignin being the predominant components. The newly produced formulas in the effluent were inferred to be associated with the anammox treatment performance. The refractory DOM, with smaller molecular weight and high reductive degree, seemed to experience a single adsorption by the stratified EPS, and became part of the anammox sludge.

AB - Anaerobic ammonium oxidation (anammox) is an energy saving and environmentally friendly technique for wastewater treatment. Sludge adsorption is an important process after organics enter the anammox reactor. The extracellular polymeric substances (EPS) of anammox sludge play a key role on the activity of anammox. This study utilized Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) to investigate molecular-level compositional characteristics of the stratified EPS of the anammox sludge, and to explore the adsorption preference of each EPS layer to refractory dissolved organic matter (DOM) during anammox treatment. Results showed that the adsorbed component by the tightly bound EPS layer was dominantly composed of lipids and proteins, with nearly 80% of formulas being CHO and CHON. The outer layers (slime, loosely bound-EPS) preferentially adsorbed the compounds with comparatively higher aromatic and unsaturated degrees, with S-containing formulas and lignin being the predominant components. The newly produced formulas in the effluent were inferred to be associated with the anammox treatment performance. The refractory DOM, with smaller molecular weight and high reductive degree, seemed to experience a single adsorption by the stratified EPS, and became part of the anammox sludge.

KW - Adsorption

KW - Anammox

KW - FT-ICR MS

KW - Refractory dissolved organic matter

KW - Stratified EPS

UR - http://www.scopus.com/inward/record.url?scp=85091197015&partnerID=8YFLogxK

U2 - 10.1016/j.energy.2020.118818

DO - 10.1016/j.energy.2020.118818

M3 - 文章

AN - SCOPUS:85091197015

SN - 0360-5442

VL - 213

JO - Energy

JF - Energy

M1 - 118818

ER -