Molecular diversity of bacterial bamA gene involved in anaerobic degradation of aromatic hydrocarbons in mesophilic petroleum reservoirs

TY - JOUR

T1 - Molecular diversity of bacterial bamA gene involved in anaerobic degradation of aromatic hydrocarbons in mesophilic petroleum reservoirs

AU - Ruan, Meng Ya

AU - Liang, Bo

AU - Mbadinga, Serge Maurice

AU - Zhou, Lei

AU - Wang, Li Ying

AU - Liu, Jing Feng

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

N1 - Publisher Copyright: © 2016 Elsevier Ltd

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Aromatic hydrocarbons are usually metabolized to the central intermediate benzoyl-coenzyme A (CoA) prior to ring cleavage. 6-Oxocyclohex-1-ene-1-carbonyl-CoA hydrolase, a key enzyme involved in the reductive benzene ring cleavage, encoded by the bamA gene can serve as a functional biomarker for detecting anaerobic degradation of aromatic compounds in different environments. In the present study, we examined the diversity the bacterial bamA gene in oil field. We sequenced bamA fragments that were PCR amplified from the microbial biomass of two mesophilic oil producing reservoirs during a period of four months. The results showed the presence and a higher diversity of bamA gene than previous studies, indicating its potential involvement in degrading aromatic hydrocarbons through anaerobic biochemical pathways in oil fields. The bamA gene sequences were affiliated to bacteria that are known to degrade aromatics, including Azoarcus, Georgfuchsia, Sulfuritalea, Peptococcaceae and Syntrophus. Differences of composition and diversity of bamA gene sequences over a period of four months in these oil reservoirs showed that it is necessary to monitor functional genes over a period of time to fully establish their significance to any specific process at any site.

AB - Aromatic hydrocarbons are usually metabolized to the central intermediate benzoyl-coenzyme A (CoA) prior to ring cleavage. 6-Oxocyclohex-1-ene-1-carbonyl-CoA hydrolase, a key enzyme involved in the reductive benzene ring cleavage, encoded by the bamA gene can serve as a functional biomarker for detecting anaerobic degradation of aromatic compounds in different environments. In the present study, we examined the diversity the bacterial bamA gene in oil field. We sequenced bamA fragments that were PCR amplified from the microbial biomass of two mesophilic oil producing reservoirs during a period of four months. The results showed the presence and a higher diversity of bamA gene than previous studies, indicating its potential involvement in degrading aromatic hydrocarbons through anaerobic biochemical pathways in oil fields. The bamA gene sequences were affiliated to bacteria that are known to degrade aromatics, including Azoarcus, Georgfuchsia, Sulfuritalea, Peptococcaceae and Syntrophus. Differences of composition and diversity of bamA gene sequences over a period of four months in these oil reservoirs showed that it is necessary to monitor functional genes over a period of time to fully establish their significance to any specific process at any site.

KW - Anaerobic aromatic ring cleavage

KW - Mesophilic

KW - Oil reservoir

KW - bamA gene

UR - http://www.scopus.com/inward/record.url?scp=84975864685&partnerID=8YFLogxK

U2 - 10.1016/j.ibiod.2016.06.005

DO - 10.1016/j.ibiod.2016.06.005

M3 - 文章

AN - SCOPUS:84975864685

SN - 0964-8305

VL - 114

SP - 122

EP - 128

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

ER -