TY - JOUR
T1 - Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation
AU - Scherr, Thomas
AU - Knapp, Gerald L.
AU - Guitreau, Amy
AU - Park, Daniel Sang Won
AU - Tiersch, Terrence
AU - Nandakumar, Krishnaswamy
AU - Monroe, W. Todd
N1 - Publisher Copyright:
© 2015, Springer Science+Business Media New York.
PY - 2015/6/2
Y1 - 2015/6/2
N2 - Sperm cell activation plays a critical role in a range of biological and engineering processes, from fertilization to cryopreservation protocol evaluation. Across a range of species, ionic and osmotic effects have been discovered that lead to activation. Sperm cells of zebrafish (Danio rerio) initiate motility in a hypoosmotic environment. In this study, we employ a microfluidic mixer for the purpose of rapidly diluting the extracellular medium to initiate the onset of cell motility. The use of a microchannel offers a rapid and reproducible mixing profile throughout the device. This greatly reduces variability from trial to trial relative to the current methods of analysis. Coupling these experiments with numerical simulations, we were able to investigate the dynamics of intracellular osmolality as each cell moves along its path through the micromixer. Our results suggest that intracellular osmolality, and hence intracellular ion concentration, only slightly decreases, contrary to the common thought that larger changes in these parameters are required for activation. Utilizing this framework, microfluidics for controlled extracellular environments and associated numerical modeling, has practical applicability in standardizing high-throughput aquatic sperm activation, and more fundamentally, investigations of the intracellular environment leading to motility.
AB - Sperm cell activation plays a critical role in a range of biological and engineering processes, from fertilization to cryopreservation protocol evaluation. Across a range of species, ionic and osmotic effects have been discovered that lead to activation. Sperm cells of zebrafish (Danio rerio) initiate motility in a hypoosmotic environment. In this study, we employ a microfluidic mixer for the purpose of rapidly diluting the extracellular medium to initiate the onset of cell motility. The use of a microchannel offers a rapid and reproducible mixing profile throughout the device. This greatly reduces variability from trial to trial relative to the current methods of analysis. Coupling these experiments with numerical simulations, we were able to investigate the dynamics of intracellular osmolality as each cell moves along its path through the micromixer. Our results suggest that intracellular osmolality, and hence intracellular ion concentration, only slightly decreases, contrary to the common thought that larger changes in these parameters are required for activation. Utilizing this framework, microfluidics for controlled extracellular environments and associated numerical modeling, has practical applicability in standardizing high-throughput aquatic sperm activation, and more fundamentally, investigations of the intracellular environment leading to motility.
KW - Microfluidic
KW - Numerical modeling
KW - Sperm analysis
KW - Transport modeling
KW - Zebrafish sperm cell activation
UR - http://www.scopus.com/inward/record.url?scp=84930193027&partnerID=8YFLogxK
U2 - 10.1007/s10544-015-9957-6
DO - 10.1007/s10544-015-9957-6
M3 - 文章
C2 - 26026298
AN - SCOPUS:84930193027
SN - 1387-2176
VL - 17
JO - Biomedical Microdevices
JF - Biomedical Microdevices
IS - 3
M1 - 65
ER -