Microbial community characteristics of petroleum reservoir production water amended with n-alkanes and incubated under nitrate-, sulfate-reducing and methanogenic conditions

TY - JOUR

T1 - Microbial community characteristics of petroleum reservoir production water amended with n-alkanes and incubated under nitrate-, sulfate-reducing and methanogenic conditions

AU - Li, Wei

AU - Wang, Li Ying

AU - Duan, Ruo Yu

AU - Liu, Jin Feng

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

N1 - Funding Information: This research was supported by the National Natural Science Foundation of China (Grant No. 51174092 ) and NSFC/RGC Joint Research Fund (No. 41161160560 ). We thank Serge Maurice Mbadinga for his suggestions and comments of this manuscript.

PY - 2012/4

Y1 - 2012/4

N2 - Methanogenic, sulfate- and nitrate-reducing enrichment cultures amended with long-chain n-alkanes (C15-C20) were established with production water from Huabei oilfield in China in the present study. Chemical analyses indicated that degradation of n-alkanes was evident under all three conditions after 356 days of incubation. Phylogenetic analyses based on 16S rRNA gene amplification indicated that α-, β-, γ-Proteobacteria and Bacteroidetes were detected in the nitrate-reducing enrichment; Actinobacteria, Nitrospira and δ-Proteobacteria were recovered from both the sulfate-reducing and methanogenic enrichments. Actinobacteria and Nitrospira were the most abundant in methanogenic and sulfate-reducing enrichment, respectively. The archaeal clone libraries showed that the order Methanomicrobiales within the phylum Euryarchaeota predominated methanogenic enrichment; whereas the unclassified class Thermoprotei within the phylum Crenarchaeota prevailed in sulfate-reducing enrichment. Comparison of 16S rRNA gene sequences from genomic DNA extracted directly from the petroleum reservoir production water with those from the three active enrichments showed that the available electron acceptors had a strong influence on the microbial community composition. In addition, genes encoding the alkylsuccinate synthase (assA) and methyl coenzyme-M reductase (mcrA) were amplified from the methanogenic enrichment and the results suggested that fumarate addition was probably involved in the degradation of n-alkanes. These results shed light on the potential utilization of microbial metabolism in remediation of hydrocarbon contamination or in enhancing the recovery of residual oil for energy.

AB - Methanogenic, sulfate- and nitrate-reducing enrichment cultures amended with long-chain n-alkanes (C15-C20) were established with production water from Huabei oilfield in China in the present study. Chemical analyses indicated that degradation of n-alkanes was evident under all three conditions after 356 days of incubation. Phylogenetic analyses based on 16S rRNA gene amplification indicated that α-, β-, γ-Proteobacteria and Bacteroidetes were detected in the nitrate-reducing enrichment; Actinobacteria, Nitrospira and δ-Proteobacteria were recovered from both the sulfate-reducing and methanogenic enrichments. Actinobacteria and Nitrospira were the most abundant in methanogenic and sulfate-reducing enrichment, respectively. The archaeal clone libraries showed that the order Methanomicrobiales within the phylum Euryarchaeota predominated methanogenic enrichment; whereas the unclassified class Thermoprotei within the phylum Crenarchaeota prevailed in sulfate-reducing enrichment. Comparison of 16S rRNA gene sequences from genomic DNA extracted directly from the petroleum reservoir production water with those from the three active enrichments showed that the available electron acceptors had a strong influence on the microbial community composition. In addition, genes encoding the alkylsuccinate synthase (assA) and methyl coenzyme-M reductase (mcrA) were amplified from the methanogenic enrichment and the results suggested that fumarate addition was probably involved in the degradation of n-alkanes. These results shed light on the potential utilization of microbial metabolism in remediation of hydrocarbon contamination or in enhancing the recovery of residual oil for energy.

KW - Enrichment cultures

KW - Functional genes

KW - Microbial communities

KW - Petroleum reservoirs

UR - http://www.scopus.com/inward/record.url?scp=84862829149&partnerID=8YFLogxK

U2 - 10.1016/j.ibiod.2012.01.005

DO - 10.1016/j.ibiod.2012.01.005

M3 - 文章

AN - SCOPUS:84862829149

SN - 0964-8305

VL - 69

SP - 87

EP - 96

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

ER -