Methanogenic biodegradation of C9 to C12 n-alkanes initiated by Smithella via fumarate addition mechanism

Jia Heng Ji; Lei Zhou; Serge Maurice Mbadinga; Muhammad Irfan; Yi Fan Liu; Pan Pan; Zhen Zhen Qi; Jing Chen; Jin Feng Liu; Shi Zhong Yang; Ji Dong Gu; Bo Zhong Mu

doi:10.1186/s13568-020-0956-5

Methanogenic biodegradation of C₉ to C₁₂ n-alkanes initiated by Smithella via fumarate addition mechanism

Jia Heng Ji, Lei Zhou, Serge Maurice Mbadinga, Muhammad Irfan, Yi Fan Liu, Pan Pan, Zhen Zhen Qi, Jing Chen, Jin Feng Liu, Shi Zhong Yang, Ji Dong Gu, Bo Zhong Mu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

In the present study, a methanogenic alkane-degrading (a mixture of C₉ to C₁₂n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over an incubation period of 1 year. At the end of the incubation, fumarate addition metabolites (C₉ to C₁₂ alkylsuccinates) and assA genes (encoding the alpha subunit of alkylsuccinate synthase) were detected only in the alkane-amended enrichment cultures. Microbial community analysis showed that putative syntrophic n-alkane degraders (Smithella) capable of initiating n-alkanes by fumarate addition mechanism were enriched in the alkane-amended enrichment cultures. In addition, both hydrogenotrophic (Methanocalculus) and acetoclastic (Methanothrix) methanogens were also observed. Our results provide further evidence that alkanes can be activated by addition to fumarate under methanogenic conditions.

Original language	English
Article number	23
Journal	AMB Express
Volume	10
Issue number	1
DOIs	https://doi.org/10.1186/s13568-020-0956-5
State	Published - 1 Dec 2020
Externally published	Yes

Keywords

Alkanes
Alkylsuccinates
Fumarate addition
Methanogenesis
Oil reservoirs

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10.1186/s13568-020-0956-5

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title = "Methanogenic biodegradation of C9 to C12 n-alkanes initiated by Smithella via fumarate addition mechanism",

abstract = "In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over an incubation period of 1 year. At the end of the incubation, fumarate addition metabolites (C9 to C12 alkylsuccinates) and assA genes (encoding the alpha subunit of alkylsuccinate synthase) were detected only in the alkane-amended enrichment cultures. Microbial community analysis showed that putative syntrophic n-alkane degraders (Smithella) capable of initiating n-alkanes by fumarate addition mechanism were enriched in the alkane-amended enrichment cultures. In addition, both hydrogenotrophic (Methanocalculus) and acetoclastic (Methanothrix) methanogens were also observed. Our results provide further evidence that alkanes can be activated by addition to fumarate under methanogenic conditions.",

keywords = "Alkanes, Alkylsuccinates, Fumarate addition, Methanogenesis, Oil reservoirs",

author = "Ji, {Jia Heng} and Lei Zhou and Mbadinga, {Serge Maurice} and Muhammad Irfan and Liu, {Yi Fan} and Pan Pan and Qi, {Zhen Zhen} and Jing Chen and Liu, {Jin Feng} and Yang, {Shi Zhong} and Gu, {Ji Dong} and Mu, {Bo Zhong}",

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doi = "10.1186/s13568-020-0956-5",

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AU - Ji, Jia Heng

AU - Zhou, Lei

AU - Mbadinga, Serge Maurice

AU - Irfan, Muhammad

AU - Liu, Yi Fan

AU - Pan, Pan

AU - Qi, Zhen Zhen

AU - Chen, Jing

AU - Liu, Jin Feng

AU - Yang, Shi Zhong

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

PY - 2020/12/1

Y1 - 2020/12/1

N2 - In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over an incubation period of 1 year. At the end of the incubation, fumarate addition metabolites (C9 to C12 alkylsuccinates) and assA genes (encoding the alpha subunit of alkylsuccinate synthase) were detected only in the alkane-amended enrichment cultures. Microbial community analysis showed that putative syntrophic n-alkane degraders (Smithella) capable of initiating n-alkanes by fumarate addition mechanism were enriched in the alkane-amended enrichment cultures. In addition, both hydrogenotrophic (Methanocalculus) and acetoclastic (Methanothrix) methanogens were also observed. Our results provide further evidence that alkanes can be activated by addition to fumarate under methanogenic conditions.

AB - In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over an incubation period of 1 year. At the end of the incubation, fumarate addition metabolites (C9 to C12 alkylsuccinates) and assA genes (encoding the alpha subunit of alkylsuccinate synthase) were detected only in the alkane-amended enrichment cultures. Microbial community analysis showed that putative syntrophic n-alkane degraders (Smithella) capable of initiating n-alkanes by fumarate addition mechanism were enriched in the alkane-amended enrichment cultures. In addition, both hydrogenotrophic (Methanocalculus) and acetoclastic (Methanothrix) methanogens were also observed. Our results provide further evidence that alkanes can be activated by addition to fumarate under methanogenic conditions.

KW - Alkanes

KW - Alkylsuccinates

KW - Fumarate addition

KW - Methanogenesis

KW - Oil reservoirs

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DO - 10.1186/s13568-020-0956-5

M3 - 文章

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ER -

Methanogenic biodegradation of C₉ to C₁₂ n-alkanes initiated by Smithella via fumarate addition mechanism

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