TY - JOUR
T1 - Intestinal paracellular permeability during malnutrition in guinea pigs
T2 - Effect of high dietary zinc
AU - Rodríguez, P.
AU - Darmon, N.
AU - Chappuis, P.
AU - Candalh, C.
AU - Blaton, M. A.
AU - Bouchaud, C.
AU - Heyman, M.
PY - 1996
Y1 - 1996
N2 - Background - Zinc has been shown to have beneficial effects in vitro on epithelial barrier function, and in vivo to reduce intestinal permeability in malnourished children with diarrhoea. Aims - To determine whether malnutrition alters intestinal paracellular permeability, and whether zinc prevents such alterations. Methods - Guinea pigs were fed a normal protein diet (NP group), a low protein diet (LP group), or a low protein diet enriched with 1800 ppm zinc (LPZn group) for three weeks. Intestinal permeability was measured on jejunal segments mounted in Ussing chambers by measuring ionic conductance and mucosal to serosal fluxes of 14C-mannitol, 22Na, and horseradish peroxidase. Tight junction morphology was assessed on cryofracture replicas. Results - Mannitol and Na fluxes and ionic conductance increased in the LP group compared with the NP group but remained normal in the LPZn group. Accordingly, jejunal epithelia from the LP group, but not from the LPZn group, showed a small decrease in number of tight junctional strands compared with epithelia from the NP group. Neither malnutrition nor zinc treatment modified horseradish peroxidase fluxes. Conclusions - Malnutrition is associated with increased intestinal paracellular permeability to small molecules, and pharmacological doses of zinc prevent such functional abnormality.
AB - Background - Zinc has been shown to have beneficial effects in vitro on epithelial barrier function, and in vivo to reduce intestinal permeability in malnourished children with diarrhoea. Aims - To determine whether malnutrition alters intestinal paracellular permeability, and whether zinc prevents such alterations. Methods - Guinea pigs were fed a normal protein diet (NP group), a low protein diet (LP group), or a low protein diet enriched with 1800 ppm zinc (LPZn group) for three weeks. Intestinal permeability was measured on jejunal segments mounted in Ussing chambers by measuring ionic conductance and mucosal to serosal fluxes of 14C-mannitol, 22Na, and horseradish peroxidase. Tight junction morphology was assessed on cryofracture replicas. Results - Mannitol and Na fluxes and ionic conductance increased in the LP group compared with the NP group but remained normal in the LPZn group. Accordingly, jejunal epithelia from the LP group, but not from the LPZn group, showed a small decrease in number of tight junctional strands compared with epithelia from the NP group. Neither malnutrition nor zinc treatment modified horseradish peroxidase fluxes. Conclusions - Malnutrition is associated with increased intestinal paracellular permeability to small molecules, and pharmacological doses of zinc prevent such functional abnormality.
KW - Guinea pig
KW - Intestine
KW - Malnutrition
KW - Paracellular permeability
KW - Tight junction
KW - Zinc
UR - http://www.scopus.com/inward/record.url?scp=0029797060&partnerID=8YFLogxK
U2 - 10.1136/gut.39.3.416
DO - 10.1136/gut.39.3.416
M3 - 文章
C2 - 8949647
AN - SCOPUS:0029797060
SN - 0017-5749
VL - 39
SP - 416
EP - 422
JO - Gut
JF - Gut
IS - 3
ER -