Highly polymorphic AFLP markers as a complementary tool to ITS sequences in assessing genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives

TY - JOUR

T1 - Highly polymorphic AFLP markers as a complementary tool to ITS sequences in assessing genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives

AU - Huang, Junchao

AU - Corke, Harold

AU - Sun, Mei

N1 - Funding Information: We thank Dr. D. Zhang of the International Potato Center (CIP) for supplying most of the plant materials used, and Dr. R. L. Jarret for providing seeds of several additional species for this study. This work was supported by research grants from the University of Hong Kong and CIP.

PY - 2002/12

Y1 - 2002/12

N2 - Comparative analyses of genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives in Ipomoea series Batatas were conducted using amplified fragment length polymorphism (AFLP) and sequence data from the internal transcribed spacer (ITS) region of the ribosomal DNA. Low ITS divergence among thirteen species of ser. Batatas resulted in poorly resolved relationships. More variable AFLP characters were found to be more efficient in characterizing genetic diversity and phylogenetic relationships at both intra- and interspecific levels within ser. Batatas. Highly informative AFLP fingerprints of 36 accessions representing 10 species of ser. Batatas were generated using only six primer combinations. Of the species examined, I. trifida was found to be the most closely related to I. batatas, while I. ramosissima and I. umbraticola were the most distantly related to I. batatas. The highly polymorphic AFLP markers are a valuable tool in assessing genetic diversity and phylogenetic relationships of sweetpotato and its wild relatives.

AB - Comparative analyses of genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives in Ipomoea series Batatas were conducted using amplified fragment length polymorphism (AFLP) and sequence data from the internal transcribed spacer (ITS) region of the ribosomal DNA. Low ITS divergence among thirteen species of ser. Batatas resulted in poorly resolved relationships. More variable AFLP characters were found to be more efficient in characterizing genetic diversity and phylogenetic relationships at both intra- and interspecific levels within ser. Batatas. Highly informative AFLP fingerprints of 36 accessions representing 10 species of ser. Batatas were generated using only six primer combinations. Of the species examined, I. trifida was found to be the most closely related to I. batatas, while I. ramosissima and I. umbraticola were the most distantly related to I. batatas. The highly polymorphic AFLP markers are a valuable tool in assessing genetic diversity and phylogenetic relationships of sweetpotato and its wild relatives.

KW - AFLP

KW - ITS

KW - Ipomoea batatas

KW - Phylogeny

KW - Sweetpotato

UR - http://www.scopus.com/inward/record.url?scp=0036915906&partnerID=8YFLogxK

U2 - 10.1023/A:1021290927362

DO - 10.1023/A:1021290927362

M3 - 文章

AN - SCOPUS:0036915906

SN - 0925-9864

VL - 49

SP - 541

EP - 550

JO - Genetic Resources and Crop Evolution

JF - Genetic Resources and Crop Evolution

IS - 6

ER -