Growth inhibition and possible mechanism of oleamide against the toxin-producing cyanobacterium Microcystis aeruginosa NIES-843

TY - JOUR

T1 - Growth inhibition and possible mechanism of oleamide against the toxin-producing cyanobacterium Microcystis aeruginosa NIES-843

AU - Shao, Jihai

AU - He, Yaxian

AU - Li, Fan

AU - Zhang, Huiling

AU - Chen, Anwei

AU - Luo, Si

AU - Gu, Ji Dong

N1 - Publisher Copyright: © 2015, Springer Science+Business Media New York.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Oleamide, a fatty acid derivative, shows inhibitory effect against the bloom-forming cyanobacterium Microcystis aeruginosa. The EC50 of oleamide on the growth of M. aeruginosa NIES-843 was 8.60 ± 1.20 mg/L. In order to elucidate the possible mechanism of toxicity of oleamide against M. aeruginosa, chlorophyll fluorescence transient, cellular ultrastructure, fatty acids composition and the transcription of the mcyB gene involved in microcystins synthesis were studied. The results of chlorophyll fluorescence transient showed that oleamide could destruct the electron accepting side of the photosystem II of M. aeruginosa NIES-843. Cellular ultrastructure examination indicated that the destruction of fatty acid constituents, the distortion of thylakoid membrane and the loss of integrity of cell membrane were associated with oleamide treatment and concentration. The damage of cellular membrane increased the release of microcystins from intact cells into the medium. Results presented in this study provide new information on the possible mechanisms involved and potential utilization of oleamide as an algicide in cyanobacterial bloom control.

AB - Oleamide, a fatty acid derivative, shows inhibitory effect against the bloom-forming cyanobacterium Microcystis aeruginosa. The EC50 of oleamide on the growth of M. aeruginosa NIES-843 was 8.60 ± 1.20 mg/L. In order to elucidate the possible mechanism of toxicity of oleamide against M. aeruginosa, chlorophyll fluorescence transient, cellular ultrastructure, fatty acids composition and the transcription of the mcyB gene involved in microcystins synthesis were studied. The results of chlorophyll fluorescence transient showed that oleamide could destruct the electron accepting side of the photosystem II of M. aeruginosa NIES-843. Cellular ultrastructure examination indicated that the destruction of fatty acid constituents, the distortion of thylakoid membrane and the loss of integrity of cell membrane were associated with oleamide treatment and concentration. The damage of cellular membrane increased the release of microcystins from intact cells into the medium. Results presented in this study provide new information on the possible mechanisms involved and potential utilization of oleamide as an algicide in cyanobacterial bloom control.

KW - Growth inhibition

KW - Microcystins

KW - Microcystis aeruginosa

KW - Oleamide

KW - Photosynthesis

UR - http://www.scopus.com/inward/record.url?scp=84955186102&partnerID=8YFLogxK

U2 - 10.1007/s10646-015-1582-x

DO - 10.1007/s10646-015-1582-x

M3 - 文章

C2 - 26547872

AN - SCOPUS:84955186102

SN - 0963-9292

VL - 25

SP - 225

EP - 233

JO - Ecotoxicology

JF - Ecotoxicology

IS - 1

ER -