Fungal diversity and its contribution to the biodeterioration of mural paintings in two 1700-year-old tombs of China

Wenxia Ma; Fasi Wu; Tian Tian; Dongpeng He; Qi Zhang; Ji Dong Gu; Yulong Duan; Dongxu Ma; Wanfu Wang; Huyuan Feng

doi:10.1016/j.ibiod.2020.104972

Fungal diversity and its contribution to the biodeterioration of mural paintings in two 1700-year-old tombs of China

Wenxia Ma, Fasi Wu, Tian Tian, Dongpeng He, Qi Zhang, Ji Dong Gu, Yulong Duan, Dongxu Ma, Wanfu Wang, Huyuan Feng^*

^*Corresponding author for this work

Environmental Science and Engineering

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Two subterranean ancient Chinese tombs of over 1700 years old, one from the Jin Dynasty and another from the Han Dynasty, were investigated in relation to black spots associated with microbiological deterioration of brick mural paintings within. The objectives of this study were to characterize the diversity of culturable fungal communities and assess the biodeteriorative potential of the isolated fungi. Culture-dependent methods using four different types of media were used for fungal isolation and specialized agar plates were used for biodeteriorative analyses by monitoring calcite dissolution, proteolytic activity, pH change, and biomineralization capabilities the isolated strains. Physical characterization of the mural samples and biomineralization were carried out using scanning electron microscopy combined with energy dispersive and X-ray diffraction analyses, while fungal acid production was analysed using High-performance liquid Chromatography (HPLC). Results showed that most of the 22 fungal strains isolated belonged to the Penicillium and Aspergillus genera. Differences in fungal community diversity between the two tombs may be attributed to variations in their interior temperature and relative humidity, however the history and drawing techniques used could also be contributing factors. Penicillium aurantiogriseum, Aspergillus versicolor and Penicillium olsonii were found in both tombs, representing the core microflora found in the black spots, thus these three fungi may be the sources of the black spots. More than 68% of the isolated fungi showed proteolytic activity and 27% of the strains produced acids, leading to dissolution of calcium carbonate and decreased pH value. Among the isolates, 5 out of 6 acid-forming fungal strains could also promote biomineralization. Penicillium was the main genus found to form acid and promote biomineralization. These fungi with biodeterioration and biomineralization characteristics were abundant in the black spots, indicating that formation of black spots was relevant to their presence and activity. These results suggest that thriving fungi, primarily driven by the local microenvironment, pose a high risk to brick wall paintings.

Original language	English
Article number	104972
Journal	International Biodeterioration and Biodegradation
Volume	152
DOIs	https://doi.org/10.1016/j.ibiod.2020.104972
State	Published - Aug 2020

Keywords

Acid production
Ancient Chinese tomb
Biodeterioration
Biomineralization
Black spot
Brick mural
Fungal disease
Fungi

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10.1016/j.ibiod.2020.104972

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title = "Fungal diversity and its contribution to the biodeterioration of mural paintings in two 1700-year-old tombs of China",

abstract = "Two subterranean ancient Chinese tombs of over 1700 years old, one from the Jin Dynasty and another from the Han Dynasty, were investigated in relation to black spots associated with microbiological deterioration of brick mural paintings within. The objectives of this study were to characterize the diversity of culturable fungal communities and assess the biodeteriorative potential of the isolated fungi. Culture-dependent methods using four different types of media were used for fungal isolation and specialized agar plates were used for biodeteriorative analyses by monitoring calcite dissolution, proteolytic activity, pH change, and biomineralization capabilities the isolated strains. Physical characterization of the mural samples and biomineralization were carried out using scanning electron microscopy combined with energy dispersive and X-ray diffraction analyses, while fungal acid production was analysed using High-performance liquid Chromatography (HPLC). Results showed that most of the 22 fungal strains isolated belonged to the Penicillium and Aspergillus genera. Differences in fungal community diversity between the two tombs may be attributed to variations in their interior temperature and relative humidity, however the history and drawing techniques used could also be contributing factors. Penicillium aurantiogriseum, Aspergillus versicolor and Penicillium olsonii were found in both tombs, representing the core microflora found in the black spots, thus these three fungi may be the sources of the black spots. More than 68% of the isolated fungi showed proteolytic activity and 27% of the strains produced acids, leading to dissolution of calcium carbonate and decreased pH value. Among the isolates, 5 out of 6 acid-forming fungal strains could also promote biomineralization. Penicillium was the main genus found to form acid and promote biomineralization. These fungi with biodeterioration and biomineralization characteristics were abundant in the black spots, indicating that formation of black spots was relevant to their presence and activity. These results suggest that thriving fungi, primarily driven by the local microenvironment, pose a high risk to brick wall paintings.",

keywords = "Acid production, Ancient Chinese tomb, Biodeterioration, Biomineralization, Black spot, Brick mural, Fungal disease, Fungi",

author = "Wenxia Ma and Fasi Wu and Tian Tian and Dongpeng He and Qi Zhang and Gu, {Ji Dong} and Yulong Duan and Dongxu Ma and Wanfu Wang and Huyuan Feng",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

month = aug,

doi = "10.1016/j.ibiod.2020.104972",

language = "英语",

volume = "152",

journal = "International Biodeterioration and Biodegradation",

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TY - JOUR

T1 - Fungal diversity and its contribution to the biodeterioration of mural paintings in two 1700-year-old tombs of China

AU - Ma, Wenxia

AU - Wu, Fasi

AU - Tian, Tian

AU - He, Dongpeng

AU - Zhang, Qi

AU - Gu, Ji Dong

AU - Duan, Yulong

AU - Ma, Dongxu

AU - Wang, Wanfu

AU - Feng, Huyuan

PY - 2020/8

Y1 - 2020/8

N2 - Two subterranean ancient Chinese tombs of over 1700 years old, one from the Jin Dynasty and another from the Han Dynasty, were investigated in relation to black spots associated with microbiological deterioration of brick mural paintings within. The objectives of this study were to characterize the diversity of culturable fungal communities and assess the biodeteriorative potential of the isolated fungi. Culture-dependent methods using four different types of media were used for fungal isolation and specialized agar plates were used for biodeteriorative analyses by monitoring calcite dissolution, proteolytic activity, pH change, and biomineralization capabilities the isolated strains. Physical characterization of the mural samples and biomineralization were carried out using scanning electron microscopy combined with energy dispersive and X-ray diffraction analyses, while fungal acid production was analysed using High-performance liquid Chromatography (HPLC). Results showed that most of the 22 fungal strains isolated belonged to the Penicillium and Aspergillus genera. Differences in fungal community diversity between the two tombs may be attributed to variations in their interior temperature and relative humidity, however the history and drawing techniques used could also be contributing factors. Penicillium aurantiogriseum, Aspergillus versicolor and Penicillium olsonii were found in both tombs, representing the core microflora found in the black spots, thus these three fungi may be the sources of the black spots. More than 68% of the isolated fungi showed proteolytic activity and 27% of the strains produced acids, leading to dissolution of calcium carbonate and decreased pH value. Among the isolates, 5 out of 6 acid-forming fungal strains could also promote biomineralization. Penicillium was the main genus found to form acid and promote biomineralization. These fungi with biodeterioration and biomineralization characteristics were abundant in the black spots, indicating that formation of black spots was relevant to their presence and activity. These results suggest that thriving fungi, primarily driven by the local microenvironment, pose a high risk to brick wall paintings.

AB - Two subterranean ancient Chinese tombs of over 1700 years old, one from the Jin Dynasty and another from the Han Dynasty, were investigated in relation to black spots associated with microbiological deterioration of brick mural paintings within. The objectives of this study were to characterize the diversity of culturable fungal communities and assess the biodeteriorative potential of the isolated fungi. Culture-dependent methods using four different types of media were used for fungal isolation and specialized agar plates were used for biodeteriorative analyses by monitoring calcite dissolution, proteolytic activity, pH change, and biomineralization capabilities the isolated strains. Physical characterization of the mural samples and biomineralization were carried out using scanning electron microscopy combined with energy dispersive and X-ray diffraction analyses, while fungal acid production was analysed using High-performance liquid Chromatography (HPLC). Results showed that most of the 22 fungal strains isolated belonged to the Penicillium and Aspergillus genera. Differences in fungal community diversity between the two tombs may be attributed to variations in their interior temperature and relative humidity, however the history and drawing techniques used could also be contributing factors. Penicillium aurantiogriseum, Aspergillus versicolor and Penicillium olsonii were found in both tombs, representing the core microflora found in the black spots, thus these three fungi may be the sources of the black spots. More than 68% of the isolated fungi showed proteolytic activity and 27% of the strains produced acids, leading to dissolution of calcium carbonate and decreased pH value. Among the isolates, 5 out of 6 acid-forming fungal strains could also promote biomineralization. Penicillium was the main genus found to form acid and promote biomineralization. These fungi with biodeterioration and biomineralization characteristics were abundant in the black spots, indicating that formation of black spots was relevant to their presence and activity. These results suggest that thriving fungi, primarily driven by the local microenvironment, pose a high risk to brick wall paintings.

KW - Acid production

KW - Ancient Chinese tomb

KW - Biodeterioration

KW - Biomineralization

KW - Black spot

KW - Brick mural

KW - Fungal disease

KW - Fungi

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DO - 10.1016/j.ibiod.2020.104972

M3 - 文章

AN - SCOPUS:85084701325

SN - 0964-8305

VL - 152

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

M1 - 104972

ER -

Fungal diversity and its contribution to the biodeterioration of mural paintings in two 1700-year-old tombs of China

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Keywords

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