TY - JOUR
T1 - Enhancing butanol tolerance and preventing degeneration in Clostridium acetobutylicum by 1-butanol-glycerol storage during long-term preservation
AU - Liu, Xiaobo
AU - Gu, Qiuya
AU - Liao, Chunli
AU - Yu, Xiaobin
N1 - Publisher Copyright:
© 2014.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - To enhance butanol tolerance and prevent productive degeneration in Clostridium acetobutylicum during long-term preservation, a novel approach called 1-butanol-glycerol storage was developed in this study. The viability, butanol tolerance and productivity of cells were investigated under each condition of storage within 12 months, respectively. The results suggested that the optimal condition of storage was in the solution containing 16 g L-1 butanol coupled with 200 g L-1 glycerol at 37 °C not -20 °C or 4 °C. Under this condition, C. acetobutylicum D64 primarily obtained the cell survival rate of 80% after 12 months. Unexpectedly, these cells still got an enhanced butanol tolerance of 32 g L-1 which was ~1 fold higher than the wild-type with butanol tolerance of 16 g L-1. Moreover, the butanol production has also got a little of increase but not dropped compared to the control. These results indicated this culture preservation method be available for enhancing butanol tolerance and preventing productive degeneration of C. acetobutylicum.
AB - To enhance butanol tolerance and prevent productive degeneration in Clostridium acetobutylicum during long-term preservation, a novel approach called 1-butanol-glycerol storage was developed in this study. The viability, butanol tolerance and productivity of cells were investigated under each condition of storage within 12 months, respectively. The results suggested that the optimal condition of storage was in the solution containing 16 g L-1 butanol coupled with 200 g L-1 glycerol at 37 °C not -20 °C or 4 °C. Under this condition, C. acetobutylicum D64 primarily obtained the cell survival rate of 80% after 12 months. Unexpectedly, these cells still got an enhanced butanol tolerance of 32 g L-1 which was ~1 fold higher than the wild-type with butanol tolerance of 16 g L-1. Moreover, the butanol production has also got a little of increase but not dropped compared to the control. These results indicated this culture preservation method be available for enhancing butanol tolerance and preventing productive degeneration of C. acetobutylicum.
KW - 1-Butanol-glycerol solution (BGS)
KW - Biofuel
KW - Butanol tolerance
KW - Clostridium acetobutylicum
KW - Long-term preservation
UR - http://www.scopus.com/inward/record.url?scp=84907330783&partnerID=8YFLogxK
U2 - 10.1016/j.biombioe.2014.07.019
DO - 10.1016/j.biombioe.2014.07.019
M3 - 文章
AN - SCOPUS:84907330783
SN - 0961-9534
VL - 69
SP - 192
EP - 197
JO - Biomass and Bioenergy
JF - Biomass and Bioenergy
ER -
