Engineering transferrable microvascular meshes for subcutaneous islet transplantation

Wei Song; Alan Chiu; Long Hai Wang; Robert E. Schwartz; Bin Li; Nikolaos Bouklas; Daniel T. Bowers; Duo An; Soon Hon Cheong; James A. Flanders; Yehudah Pardo; Qingsheng Liu; Xi Wang; Vivian K. Lee; Guohao Dai; Minglin Ma

doi:10.1038/s41467-019-12373-5

Engineering transferrable microvascular meshes for subcutaneous islet transplantation

Wei Song, Alan Chiu, Long Hai Wang, Robert E. Schwartz, Bin Li, Nikolaos Bouklas, Daniel T. Bowers, Duo An, Soon Hon Cheong, James A. Flanders, Yehudah Pardo, Qingsheng Liu, Xi Wang, Vivian K. Lee, Guohao Dai, Minglin Ma^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

The success of engineered cell or tissue implants is dependent on vascular regeneration to meet adequate metabolic requirements. However, development of a broadly applicable strategy for stable and functional vascularization has remained challenging. We report here highly organized and resilient microvascular meshes fabricated through a controllable anchored self-assembly method. The microvascular meshes are scalable to centimeters, almost free of defects and transferrable to diverse substrates, ready for transplantation. They promote formation of functional blood vessels, with a density as high as ~220 vessels mm^-2, in the poorly vascularized subcutaneous space of SCID-Beige mice. We further demonstrate the feasibility of fabricating microvascular meshes from human induced pluripotent stem cell-derived endothelial cells, opening a way to engineer patient-specific microvasculature. As a proof-of-concept for type 1 diabetes treatment, we combine microvascular meshes and subcutaneously transplanted rat islets and achieve correction of chemically induced diabetes in SCID-Beige mice for 3 months.

Original language	English
Article number	4602
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-12373-5
State	Published - 1 Dec 2019
Externally published	Yes

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title = "Engineering transferrable microvascular meshes for subcutaneous islet transplantation",

abstract = "The success of engineered cell or tissue implants is dependent on vascular regeneration to meet adequate metabolic requirements. However, development of a broadly applicable strategy for stable and functional vascularization has remained challenging. We report here highly organized and resilient microvascular meshes fabricated through a controllable anchored self-assembly method. The microvascular meshes are scalable to centimeters, almost free of defects and transferrable to diverse substrates, ready for transplantation. They promote formation of functional blood vessels, with a density as high as ~220 vessels mm-2, in the poorly vascularized subcutaneous space of SCID-Beige mice. We further demonstrate the feasibility of fabricating microvascular meshes from human induced pluripotent stem cell-derived endothelial cells, opening a way to engineer patient-specific microvasculature. As a proof-of-concept for type 1 diabetes treatment, we combine microvascular meshes and subcutaneously transplanted rat islets and achieve correction of chemically induced diabetes in SCID-Beige mice for 3 months.",

author = "Wei Song and Alan Chiu and Wang, {Long Hai} and Schwartz, {Robert E.} and Bin Li and Nikolaos Bouklas and Bowers, {Daniel T.} and Duo An and Cheong, {Soon Hon} and Flanders, {James A.} and Yehudah Pardo and Qingsheng Liu and Xi Wang and Lee, {Vivian K.} and Guohao Dai and Minglin Ma",

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doi = "10.1038/s41467-019-12373-5",

language = "英语",

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T1 - Engineering transferrable microvascular meshes for subcutaneous islet transplantation

AU - Song, Wei

AU - Chiu, Alan

AU - Wang, Long Hai

AU - Schwartz, Robert E.

AU - Li, Bin

AU - Bouklas, Nikolaos

AU - Bowers, Daniel T.

AU - An, Duo

AU - Cheong, Soon Hon

AU - Flanders, James A.

AU - Pardo, Yehudah

AU - Liu, Qingsheng

AU - Wang, Xi

AU - Lee, Vivian K.

AU - Dai, Guohao

AU - Ma, Minglin

PY - 2019/12/1

Y1 - 2019/12/1

N2 - The success of engineered cell or tissue implants is dependent on vascular regeneration to meet adequate metabolic requirements. However, development of a broadly applicable strategy for stable and functional vascularization has remained challenging. We report here highly organized and resilient microvascular meshes fabricated through a controllable anchored self-assembly method. The microvascular meshes are scalable to centimeters, almost free of defects and transferrable to diverse substrates, ready for transplantation. They promote formation of functional blood vessels, with a density as high as ~220 vessels mm-2, in the poorly vascularized subcutaneous space of SCID-Beige mice. We further demonstrate the feasibility of fabricating microvascular meshes from human induced pluripotent stem cell-derived endothelial cells, opening a way to engineer patient-specific microvasculature. As a proof-of-concept for type 1 diabetes treatment, we combine microvascular meshes and subcutaneously transplanted rat islets and achieve correction of chemically induced diabetes in SCID-Beige mice for 3 months.

AB - The success of engineered cell or tissue implants is dependent on vascular regeneration to meet adequate metabolic requirements. However, development of a broadly applicable strategy for stable and functional vascularization has remained challenging. We report here highly organized and resilient microvascular meshes fabricated through a controllable anchored self-assembly method. The microvascular meshes are scalable to centimeters, almost free of defects and transferrable to diverse substrates, ready for transplantation. They promote formation of functional blood vessels, with a density as high as ~220 vessels mm-2, in the poorly vascularized subcutaneous space of SCID-Beige mice. We further demonstrate the feasibility of fabricating microvascular meshes from human induced pluripotent stem cell-derived endothelial cells, opening a way to engineer patient-specific microvasculature. As a proof-of-concept for type 1 diabetes treatment, we combine microvascular meshes and subcutaneously transplanted rat islets and achieve correction of chemically induced diabetes in SCID-Beige mice for 3 months.

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DO - 10.1038/s41467-019-12373-5

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AN - SCOPUS:85073099809

SN - 2041-1723

VL - 10

JO - Nature Communications

JF - Nature Communications

IS - 1

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ER -

Engineering transferrable microvascular meshes for subcutaneous islet transplantation

Abstract

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