Elimination of the formation of biofilm in industrial pipes using enzyme cleaning technique

Xiaobo Liu; Bo Tang; Qiuya Gu; Xiaobin Yu

doi:10.1016/j.mex.2014.08.008

Elimination of the formation of biofilm in industrial pipes using enzyme cleaning technique

Xiaobo Liu^*, Bo Tang, Qiuya Gu, Xiaobin Yu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Currently, there is a growing demand in how to eliminate the biofilm formed in industrial pipelines, especially in food, fermentation, and water treatment industry. However, the traditional techniques for CIP (cleaning in place) are usually ineffective, superficial, halfway, and do not clean or sterilize microbes located in the inner layers of the biofilm. A recent strategy for removing the biofilm in pipes is employing enzymes to clean it in the circulating water system under an optimal condition. However, how to operate and control the whole cleaning process is difficult. Here, we will introduce the strategy of enzyme cleaning to make it more appropriated and effective.A modification of CIP method is proposed for higher efficiency by using N-acetylmuramide glycanohydrolase as catalysts whose optimal pH and temperature is 10 ± 1 and 45 ± 2 °C, respectively.The initial efficiency of enzyme cleaning was evaluated by testing the content of ATP in water sample using Clean-Trace™ (3M Corporation).Lastly, the terminal water was tested with SLYM-BART™ (HACH Corporation) to find out whether there were biofilm-forming bacteria, such as Pseudomonas aeruginosa (Lakretz et al. (2011) [1]), Pseudomonas fluorescens (O'Toole and Kolter (1998) [2]), iron bacterium, etc. MethodEnzyme cleaning technique.

Original language	English
Pages (from-to)	e130-e136
Journal	MethodsX
Volume	1
DOIs	https://doi.org/10.1016/j.mex.2014.08.008
State	Published - 2014
Externally published	Yes

Keywords

Biofilm
Biofilm-forming
CIP (cleaning in place)
Enzyme cleaning
bacteria

Access to Document

10.1016/j.mex.2014.08.008

Cite this

@article{90a13b6dd59c4b159d47d8a1184c7af6,

title = "Elimination of the formation of biofilm in industrial pipes using enzyme cleaning technique",

abstract = "Currently, there is a growing demand in how to eliminate the biofilm formed in industrial pipelines, especially in food, fermentation, and water treatment industry. However, the traditional techniques for CIP (cleaning in place) are usually ineffective, superficial, halfway, and do not clean or sterilize microbes located in the inner layers of the biofilm. A recent strategy for removing the biofilm in pipes is employing enzymes to clean it in the circulating water system under an optimal condition. However, how to operate and control the whole cleaning process is difficult. Here, we will introduce the strategy of enzyme cleaning to make it more appropriated and effective.A modification of CIP method is proposed for higher efficiency by using N-acetylmuramide glycanohydrolase as catalysts whose optimal pH and temperature is 10 ± 1 and 45 ± 2 °C, respectively.The initial efficiency of enzyme cleaning was evaluated by testing the content of ATP in water sample using Clean-Trace{\texttrademark} (3M Corporation).Lastly, the terminal water was tested with SLYM-BART{\texttrademark} (HACH Corporation) to find out whether there were biofilm-forming bacteria, such as Pseudomonas aeruginosa (Lakretz et al. (2011) [1]), Pseudomonas fluorescens (O'Toole and Kolter (1998) [2]), iron bacterium, etc. MethodEnzyme cleaning technique.",

keywords = "Biofilm, Biofilm-forming, CIP (cleaning in place), Enzyme cleaning, bacteria",

author = "Xiaobo Liu and Bo Tang and Qiuya Gu and Xiaobin Yu",

note = "Publisher Copyright: {\textcopyright} 2014 The Authors.",

year = "2014",

doi = "10.1016/j.mex.2014.08.008",

language = "英语",

volume = "1",

pages = "e130--e136",

journal = "MethodsX",

issn = "2215-0161",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Elimination of the formation of biofilm in industrial pipes using enzyme cleaning technique

AU - Liu, Xiaobo

AU - Tang, Bo

AU - Gu, Qiuya

AU - Yu, Xiaobin

PY - 2014

Y1 - 2014

N2 - Currently, there is a growing demand in how to eliminate the biofilm formed in industrial pipelines, especially in food, fermentation, and water treatment industry. However, the traditional techniques for CIP (cleaning in place) are usually ineffective, superficial, halfway, and do not clean or sterilize microbes located in the inner layers of the biofilm. A recent strategy for removing the biofilm in pipes is employing enzymes to clean it in the circulating water system under an optimal condition. However, how to operate and control the whole cleaning process is difficult. Here, we will introduce the strategy of enzyme cleaning to make it more appropriated and effective.A modification of CIP method is proposed for higher efficiency by using N-acetylmuramide glycanohydrolase as catalysts whose optimal pH and temperature is 10 ± 1 and 45 ± 2 °C, respectively.The initial efficiency of enzyme cleaning was evaluated by testing the content of ATP in water sample using Clean-Trace™ (3M Corporation).Lastly, the terminal water was tested with SLYM-BART™ (HACH Corporation) to find out whether there were biofilm-forming bacteria, such as Pseudomonas aeruginosa (Lakretz et al. (2011) [1]), Pseudomonas fluorescens (O'Toole and Kolter (1998) [2]), iron bacterium, etc. MethodEnzyme cleaning technique.

AB - Currently, there is a growing demand in how to eliminate the biofilm formed in industrial pipelines, especially in food, fermentation, and water treatment industry. However, the traditional techniques for CIP (cleaning in place) are usually ineffective, superficial, halfway, and do not clean or sterilize microbes located in the inner layers of the biofilm. A recent strategy for removing the biofilm in pipes is employing enzymes to clean it in the circulating water system under an optimal condition. However, how to operate and control the whole cleaning process is difficult. Here, we will introduce the strategy of enzyme cleaning to make it more appropriated and effective.A modification of CIP method is proposed for higher efficiency by using N-acetylmuramide glycanohydrolase as catalysts whose optimal pH and temperature is 10 ± 1 and 45 ± 2 °C, respectively.The initial efficiency of enzyme cleaning was evaluated by testing the content of ATP in water sample using Clean-Trace™ (3M Corporation).Lastly, the terminal water was tested with SLYM-BART™ (HACH Corporation) to find out whether there were biofilm-forming bacteria, such as Pseudomonas aeruginosa (Lakretz et al. (2011) [1]), Pseudomonas fluorescens (O'Toole and Kolter (1998) [2]), iron bacterium, etc. MethodEnzyme cleaning technique.

KW - Biofilm

KW - Biofilm-forming

KW - CIP (cleaning in place)

KW - Enzyme cleaning

KW - bacteria

UR - http://www.scopus.com/inward/record.url?scp=84907481367&partnerID=8YFLogxK

U2 - 10.1016/j.mex.2014.08.008

DO - 10.1016/j.mex.2014.08.008

M3 - 文章

AN - SCOPUS:84907481367

SN - 2215-0161

VL - 1

SP - e130-e136

JO - MethodsX

JF - MethodsX

ER -

Elimination of the formation of biofilm in industrial pipes using enzyme cleaning technique

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this