Dominance of Pseudomonas in bacterial community and inhibition of fumarate addition pathway by injection of nutrients in oil reservoir revealed by functional gene and their transcript analyses

TY - JOUR

T1 - Dominance of Pseudomonas in bacterial community and inhibition of fumarate addition pathway by injection of nutrients in oil reservoir revealed by functional gene and their transcript analyses

AU - Liu, Jin Feng

AU - Lu, Yu Wei

AU - Liu, Xiao Bo

AU - Li, Bai Guang

AU - Sun, Yu Fei

AU - Zhou, Lei

AU - Liu, Yi Fan

AU - Yang, Shi Zhong

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

N1 - Publisher Copyright: © 2020

PY - 2020/9

Y1 - 2020/9

N2 - The microbial community composition in subsurface oil reservoirs is responsible for the biogeochemical reactions of hydrocarbon transformation. In this study, the composition, major functions and gene expression activity of the microorganisms in production water from an oil reservoir were analyzed prior to and after injection nutrients using high-throughput 16S rRNA gene sequencing, functional gene and their transcript profiling. Pseudomonas spp. was the dominant bacteria showing a quick increase in the bacterial population. The injection of nutrients lowered the activity of assA-bearing bacteria, but accelerated the activity of methanogens. The results show that the anaerobic hydrocarbon degradation was actively occurring in situ oil reservoir sampled, Smithella was the active anaerobic hydrocarbon degrading bacteria, and Methanoculleus and Methanothrix were the actively dominant methanogens. The present study provides a snapshot of the practical technology with insightful results to support the research, development and utilization of reservoir through biological augmentation.

AB - The microbial community composition in subsurface oil reservoirs is responsible for the biogeochemical reactions of hydrocarbon transformation. In this study, the composition, major functions and gene expression activity of the microorganisms in production water from an oil reservoir were analyzed prior to and after injection nutrients using high-throughput 16S rRNA gene sequencing, functional gene and their transcript profiling. Pseudomonas spp. was the dominant bacteria showing a quick increase in the bacterial population. The injection of nutrients lowered the activity of assA-bearing bacteria, but accelerated the activity of methanogens. The results show that the anaerobic hydrocarbon degradation was actively occurring in situ oil reservoir sampled, Smithella was the active anaerobic hydrocarbon degrading bacteria, and Methanoculleus and Methanothrix were the actively dominant methanogens. The present study provides a snapshot of the practical technology with insightful results to support the research, development and utilization of reservoir through biological augmentation.

KW - Evolution

KW - Functional gene

KW - Microbial composition

KW - Nutrient injection

KW - Oil reservoir

KW - Transcripts

UR - http://www.scopus.com/inward/record.url?scp=85087284993&partnerID=8YFLogxK

U2 - 10.1016/j.ibiod.2020.105039

DO - 10.1016/j.ibiod.2020.105039

M3 - 文章

AN - SCOPUS:85087284993

SN - 0964-8305

VL - 153

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

M1 - 105039

ER -